Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
C184S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C24S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C269S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C270S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C273S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C332S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C347S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
C80S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
H34Q | enzyme is totally inactive | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuriphenylsulfonic acid | - |
Trypanosoma brucei | |
Zn2+ | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | 0.0007 | Variant-surface-glycoprotein | pH 8 | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
- |
Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
variant-surface-glycoprotein | - |
Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | - |
? |