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Literature summary for 4.6.1.1 extracted from

  • Brown, J.T.; Kant, A.; Mailman, R.B.
    Rapid, semi-automated, and inexpensive radioimmunoassay of cAMP: Application in GPCR-mediated adenylate cyclase assays (2009), J. Neurosci. Methods, 177, 261-266.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Homo sapiens
-
3',5'-cyclic-AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell HEK-hD1, HEK-293 cells transiently transfected with human D1 dopamine receptor Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Homo sapiens 3',5'-cyclic-AMP + diphosphate
-
?
additional information optimization of a radioimmunoassay method for cAMP, with markedly improved speed and reduced cost without sacrificing sensitivity, and also adaptable to analysis of cGMP. Development of a simple, reproducible, and inexpensive method to make the radiomarker used for the radioimmunoassay, overview Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens