Activating Compound | Comment | Organism | Structure |
---|---|---|---|
forskolin | - |
Rattus norvegicus | |
forskolin | stimulates Gbetagamma subunits whereby enhancing the activity of enzymes | Homo sapiens | |
forskolin | stimulates the activity of soluble forms of ACV comprising their C1 and C2 domains, but Gbeta1gamma2 does not alter the ability of the agonist to regulate adenylate cyclase activity | Canis lupus familiaris | |
Galphas | - |
Rattus norvegicus | |
Galphas | stimulates Gbetagamma subunits whereby enhancing the activity of enzymes | Homo sapiens | |
Galphas | stimulates the activity of soluble forms of ACV comprising their C1 and C2 domains, but Gbeta1gamma2 does not alter the ability of the agonist to regulate adenylate cyclase activity | Canis lupus familiaris | |
Gbeta1gamma2 | increases the activation of ACV and ACVI by forskolin or Galphas. Gbetagamma subunits derived from sources other than Gi participate in mediating the full activation of ACVI by the beta-adrenergic receptor agonist, isoproterenol | Homo sapiens | |
Gbetagamma | conditionally stimulates ACV and ACVI | Rattus norvegicus | |
Gbetagamma | Gbetagamma conditionally stimulates ACVI, but does not stimulate ACV | Canis lupus familiaris |
Cloned (Comment) | Organism |
---|---|
ACV expressed in Sf9 cells | Canis lupus familiaris |
ACV fused with a GST tag and expressed in Escherichia coli, wild-type and mutant ACVIdeltaN enzymes expressed in Sf9 cells, COS-7 cells overexpressing ACVI, binding domain vector pGBKT7 together with pGADT7-beta1 or the activating vector pGADT7 together with pGBKT7-N-terminal region of ACV or ACVI co-transformed into AH109 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant ACVIdeltaN, shows a much lower activity as compared with full-length ACVI, but its stimulated activity is about 4fold higher than the corresponding control activity, activities of the recombinant, engineered, soluble forms of ACV and ACVI, which lack the N termini, are not enhanced by Gbetagamma subunits. Deletion of residues 77-151, but not 1-76, in the N-terminal region of ACVI obliterates the ability of Gbetagamma subunits to conditionally stimulate the enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Galphai | inhibits Galphas-stimulated activities of ACVI, Gbetagamma does not alter the ability of Galphai to inhibit the activities of ACVI | Homo sapiens | |
additional information | G protein coupled receptor kinase 2 by sequestering Gbetagamma subunits in vivo decreases isoproterenol-elicited increase in cAMP accumulation in cells expressing ACVI. Pertussis toxin treatment does not alter the ability of G protein coupled receptor kinase 2 to attenuate isoproterenol-stimulated ACVI activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ACV purified by gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Homo sapiens | 3',5'-cAMP + diphosphate | - |
? | |
ATP | - |
Rattus norvegicus | 3',5'-cAMP + diphosphate | - |
? | |
ATP | - |
Canis lupus familiaris | 3',5'-cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACV | - |
Homo sapiens |
ACV | - |
Rattus norvegicus |
ACV | - |
Canis lupus familiaris |
ACVI | - |
Homo sapiens |
ACVI | - |
Rattus norvegicus |
ACVI | - |
Canis lupus familiaris |
type VI adenylyl cyclase | - |
Homo sapiens |