Cloned (Comment) | Organism |
---|---|
cloned into pET15b vector, recombinant plasmid isolated from Escherichia coli NovaBlue cells and introduced into Escherichia coli BL21(DE3) cells for protein expression | Yersinia pestis |
Crystallization (Comment) | Organism |
---|---|
by vapour diffusion-hanging drop method, amino-terminal His-tagged construct, from which the tag was removed by thrombin, crystallized in a triclinic form diffracting to 1.9 A resolution, with one dimer per asymmetric unit and unit-cell parameters a = 33.5, b = 35.5, c = 71.8 A, alpha = 88.7, beta = 82.5, gamma = 65.5°. Non-His tagged native construct crystallized in space group P212121 with unit-cell parameters a = 56.8, b = 118.6, c = 144.5 A, to 2.9 A resolution and probably having two dimers per asymmetric unit | Yersinia pestis |
Protein Variants | Comment | Organism |
---|---|---|
D143A | does not crystallize | Yersinia pestis |
E10A | gives crystals that are unsuitable owing to poor crystal growth or poor diffraction | Yersinia pestis |
E136A | gives crystals that are unsuitable owing to poor crystal growth or poor diffraction | Yersinia pestis |
E84A | gives crystals that are unsuitable owing to poor crystal growth or poor diffraction | Yersinia pestis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20500 | - |
non-His tagged native construct | Yersinia pestis |
40000 | - |
gel filtration | Yersinia pestis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
non-His tagged native construct, on NiNTA resin and by gel filtration | Yersinia pestis |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Yersinia pestis |
Synonyms | Comment | Organism |
---|---|---|
class IV adenylyl cyclase | - |
Yersinia pestis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
crystal forms of the native and His tagged enzyme appear to require pH below 5 | Yersinia pestis |