Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated version of Cya1 consisting of amino acids 95 to 338, mutant with significant lower specific activity | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | NaCl, KCl not inhibitory | Synechocystis sp. | |
NaHCO3 | approximately 50% inhibition of enzyme activity by 50 mM NaHCO3 | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
ATP | native Cya1, pH 7.5, 27°C | Synechocystis sp. | |
0.0027 | - |
ATP | truncated Cya1, pH 7.5, 27°C | Synechocystis sp. | |
0.0327 | - |
ATP | truncated Cya1, pH 7.5, 27°C, 50 mM NaHCO3 | Synechocystis sp. | |
0.0339 | - |
ATP | native Cya1, pH 7.5, 27°C, 50 mM NaHCO3 | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803 | - |
Purification (Comment) | Organism |
---|---|
- |
Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
native Cya1, Vmax 65.7 pmol cAMP/min/nmol protein, pH 7.5, 27°C, Vmax 40.5 pmol cAMP/min/nmol protein, pH 7.5, 27°C, 50 mM NaHCO3 | Synechocystis sp. |
additional information | - |
truncated Cya1, Vmax 17.6 pmol cAMP/min/nmol protein, pH 7.5, 27°C, Vmax 10.4 pmol cAMP/min/nmol protein, pH 7.5, 27°C, 50 mM NaHCO3 | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Synechocystis sp. | 3',5'-cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Synechocystis sp. |
Cya1 | - |
Synechocystis sp. |