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Literature summary for 4.6.1.1 extracted from

  • Masuda, S.; Ono, T.a.
    Adenylyl cyclase activity of Cya1 from the cyanobacterium Synechocystis sp. strain PCC 6803 is inhibited by bicarbonate (2005), J. Bacteriol., 187, 5032-5035.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information truncated version of Cya1 consisting of amino acids 95 to 338, mutant with significant lower specific activity Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information NaCl, KCl not inhibitory Synechocystis sp.
NaHCO3 approximately 50% inhibition of enzyme activity by 50 mM NaHCO3 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
ATP native Cya1, pH 7.5, 27°C Synechocystis sp.
0.0027
-
ATP truncated Cya1, pH 7.5, 27°C Synechocystis sp.
0.0327
-
ATP truncated Cya1, pH 7.5, 27°C, 50 mM NaHCO3 Synechocystis sp.
0.0339
-
ATP native Cya1, pH 7.5, 27°C, 50 mM NaHCO3 Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
-
Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
native Cya1, Vmax 65.7 pmol cAMP/min/nmol protein, pH 7.5, 27°C, Vmax 40.5 pmol cAMP/min/nmol protein, pH 7.5, 27°C, 50 mM NaHCO3 Synechocystis sp.
additional information
-
truncated Cya1, Vmax 17.6 pmol cAMP/min/nmol protein, pH 7.5, 27°C, Vmax 10.4 pmol cAMP/min/nmol protein, pH 7.5, 27°C, 50 mM NaHCO3 Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Synechocystis sp. 3',5'-cAMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
adenylyl cyclase
-
Synechocystis sp.
Cya1
-
Synechocystis sp.