Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
H351A | mutant with no enzyme activity | Bacillus anthracis |
H351F | mutant with 40fold decreased enzyme activity | Bacillus anthracis |
H351N | mutant with 34fold decreased enzyme activity | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-dATP | competitive inhibition | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | wild type, pH 8.0, 25°C | Bacillus anthracis | |
0.17 | - |
ATP | mutant H351N, pH 8.0, 25°C | Bacillus anthracis | |
0.19 | - |
ATP | mutant H351F, pH 8.0, 25°C | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Bacillus anthracis | 3',5'-cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Edema factor | - |
Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
wild type and mutants H351A, H351N, H351F | Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.104 | - |
3'-dATP | wild type | Bacillus anthracis | |
0.132 | - |
3'-dATP | mutant H351N | Bacillus anthracis | |
0.146 | - |
3'-dATP | mutant H351F | Bacillus anthracis |