Literature summary for 4.6.1.1 extracted from

Kasahara, M.; Unno, T.; Yashiro, K.; Ohmori, M.
CyaG, a novel cyanobacterial adenylyl cyclase and a possible ancestor of mammalian guanylyl cyclases (2001), J. Biol. Chem., 276, 10564-10569.

Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminal domain as GST-fusion protein in Escherichia coli	Arthrospira platensis

Protein Variants

Protein Variants	Comment	Organism
K533E	no activity	Arthrospira platensis
K533E/I603R/D605C	mutant lose adenylyl cyclase activity but obtains significant guanylyl cyclase activity	Arthrospira platensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Arthrospira platensis	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	1 mM, strong activation	Arthrospira platensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP	Arthrospira platensis	-	3',5'-cyclic AMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrospira platensis	Q9EXQ2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminal domain	Arthrospira platensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00036	-	in the presence of 1 mM Mg2+, recombinant C-terminal CyaG domain	Arthrospira platensis
0.011	-	in the presence of 1 mM Mn2+, recombinant C-terminal CyaG domain	Arthrospira platensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Arthrospira platensis	3',5'-cyclic AMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
adenylyl cyclase	-	Arthrospira platensis
CyaG	-	Arthrospira platensis

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Release 2023.1 (January 2023)