Literature summary for 4.5.1.3 extracted from

Yu, J.; Liu, Q.; Liu, L.; Chen, J.
Cloning and characterization of dichloromethane dehalogenase from Methylobacterium rhodesianum for dichloromethane degradation (2017), Bioremediat. J., 21, 71-80 .

No PubMed abstract available

Search for more literature for 4.5.1.3

Application

Application	Comment	Organism
degradation	enzyme activity in recombinant cells is 3 times higher than that in the wild-type Methylorubrum rhodesianum. Degradation efficiency of dichloromethane reaches 86.11% within 20 h and is highly associated with glutathione concentration	Methylorubrum rhodesianum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methylorubrum rhodesianum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10.96	-	Dichloromethane	pH 7.0, 40°C	Methylorubrum rhodesianum

Organism

Organism	UniProt	Comment	Textmining
Methylorubrum rhodesianum	A0A088MAR6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Methylorubrum rhodesianum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dichloromethane + H2O	-	Methylorubrum rhodesianum	formaldehyde + 2 Cl-	-	?

Subunits

Subunits	Comment	Organism
?	x * 33058, calculated from sequence, x * 33000, SDS-PAGE	Methylorubrum rhodesianum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Methylorubrum rhodesianum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	8	-	Methylorubrum rhodesianum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Methylorubrum rhodesianum	calculated from sequence	-	5.1

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Release 2023.1 (January 2023)