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Literature summary for 4.5.1.3 extracted from

  • Firsova, Y.; Fedorov, D.; Trotsenko, Y.
    Analysis of the 3'-region of the dcmA gene of dichloromethane dehalogenase of Methylobacterium dichloromethanicum DM4 (2011), Microbiology, 80, 805-811.
No PubMed abstract available
Search for more literature for 4.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
cloning and sequencing of gene dcmA Methylorubrum extorquens
cloning and sequencing of gene METDI2657 located in the 3'-region of the dcmA gene of DCM dehalogenase, RT-PCR expression analysis Methylorubrum extorquens
cloning and sequencing of gene METDI2658, located in the 3'-region of the dcmA gene of DCM dehalogenase, RT-PCR expression analysis of the gene METDI2658 Methylorubrum extorquens

Protein Variants

Protein Variants Comment Organism
additional information generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme Methylorubrum extorquens

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens P21161 gene dcmA or METDI2656; gene dcmA
-
Methylorubrum extorquens Q50402 gene METDI2657; gene METDI2657
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Methylorubrum extorquens Q50403 gene METDI2658; gene METDI2658
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Source Tissue

Source Tissue Comment Organism Textmining
culture condition:dichloromethane-grown cell expression of gene dcmA Methylorubrum extorquens
-
culture condition:dichloromethane-grown cell expression of gene METDI2657 Methylorubrum extorquens
-
culture condition:dichloromethane-grown cell expression of gene METDI2658 Methylorubrum extorquens
-
culture condition:methanol-grown cell expression of gene dcmA Methylorubrum extorquens
-
culture condition:methanol-grown cell expression of gene METDI2657 Methylorubrum extorquens
-
culture condition:methanol-grown cell expression of gene METDI2658 Methylorubrum extorquens
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Synonyms

Synonyms Comment Organism
DCM dehalogenase
-
 Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methylorubrum extorquens