Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Methylophilus sp. |
Cloned (Comment) | Organism |
---|---|
- |
Methylophilus sp. |
Protein Variants | Comment | Organism |
---|---|---|
H8F | little effect on the activity, but slower turnover of dibromomethane than wild-type | Methylophilus sp. |
H8L | little effect on the activity, but slower turnover of dibromomethane than wild-type | Methylophilus sp. |
H8Y | little effect on the activity, but slower turnover of dibromomethane than wild-type | Methylophilus sp. |
S12A | drastically reduced activity | Methylophilus sp. |
S12T | drastically reduced activity | Methylophilus sp. |
Y6A | little effect on the activity | Methylophilus sp. |
Y6F | little effect on the activity | Methylophilus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
Dibromomethane | 30°C, pH 8.5, Y6A | Methylophilus sp. | |
0.001 | - |
Dibromomethane | 30°C, pH 8.5, H8F | Methylophilus sp. | |
0.0029 | - |
Dibromomethane | 30°C, pH 8.5, Y6F | Methylophilus sp. | |
0.0032 | - |
Dibromomethane | 30°C, pH 8.5, H8Y | Methylophilus sp. | |
0.0035 | - |
Dibromomethane | 30°C, pH 8.5, wild-type | Methylophilus sp. | |
0.032 | - |
Dichloromethane | 30°C, pH 8.5, Y6F | Methylophilus sp. | |
0.034 | - |
Dichloromethane | 30°C, pH 8.5, Y6A | Methylophilus sp. | |
0.049 | - |
Dichloromethane | 30°C, pH 8.5, H8F | Methylophilus sp. | |
0.055 | - |
Dichloromethane | 30°C, pH 8.5, H8Y | Methylophilus sp. | |
0.059 | - |
Dichloromethane | 30°C, pH 8.5, wild-type | Methylophilus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dichloromethane + H2O | Methylophilus sp. | - |
formaldehyde + Cl- | - |
? | |
dichloromethane + H2O | Methylophilus sp. DM11 | - |
formaldehyde + Cl- | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus sp. | P43387 | - |
- |
Methylophilus sp. DM11 | P43387 | - |
- |
Purification (Comment) | Organism |
---|---|
strain DM11 | Methylophilus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibromomethane + H2O | - |
Methylophilus sp. | formaldehyde + Br- | - |
? | |
dibromomethane + H2O | - |
Methylophilus sp. DM11 | formaldehyde + Br- | - |
? | |
dichloromethane + H2O | - |
Methylophilus sp. | formaldehyde + Cl- | - |
? | |
dichloromethane + H2O | - |
Methylophilus sp. DM11 | formaldehyde + Cl- | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
Dichloromethane | 30°C, pH 8.5, Y6A | Methylophilus sp. | |
2.6 | - |
Dichloromethane | 30°C, pH 8.5, Y6F | Methylophilus sp. | |
3 | - |
Dichloromethane | 30°C, pH 8.5, H8Y | Methylophilus sp. | |
3 | - |
Dibromomethane | 30°C, pH 8.5, H8Y | Methylophilus sp. | |
3.3 | - |
Dichloromethane | 30°C, pH 8.5, wild-type | Methylophilus sp. | |
3.4 | - |
Dibromomethane | 30°C, pH 8.5, H8F | Methylophilus sp. | |
3.6 | - |
Dichloromethane | 30°C, pH 8.5, H8F | Methylophilus sp. | |
4.4 | - |
Dibromomethane | 30°C, pH 8.5, Y6A | Methylophilus sp. | |
5.5 | - |
Dibromomethane | 30°C, pH 8.5, wild-type | Methylophilus sp. | |
5.6 | - |
Dibromomethane | 30°C, pH 8.5, Y6F | Methylophilus sp. |