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Literature summary for 4.5.1.2 extracted from

  • Nagasawa, T.; Ohkishi, H.; Kavakami, B.; Yamano, H.; Hosono, H.; Tani, Y.; Yamada, H.
    3-Chloro-D-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1.1. Purification and characterization of a novel enzyme occurring in 3-chloro-D-alanine-resistant pseudomonads (1982), J. Biol. Chem., 257, 13749-13756.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Pseudomonas putida
CuSO3
-
 Pseudomonas putida
cycloserine
-
 Pseudomonas putida
cysteamine dihydrochloride
-
 Pseudomonas putida
D-penicillamine
-
 Pseudomonas putida
HgCl2
-
 Pseudomonas putida
hydroxylamine HCl
-
 Pseudomonas putida
iodoacetate
-
 Pseudomonas putida
KCN
-
 Pseudomonas putida
L-Penicillamine
-
 Pseudomonas putida
phenylhydrazine
-
 Pseudomonas putida
Semicarbazide hydrochloride
-
 Pseudomonas putida
thiol reagents
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 3-chloro-D-alanine
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Pseudomonas putida
76000
-
 sedimentation equilibrium Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 CR1-1, AKU 867
-

Purification (Commentary)

Purification (Comment) Organism
CR1-1 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min stable Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 2 mol pyridoxal 5'-phosphate per mol of enzyme Pseudomonas putida