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Literature summary for 4.4.1.9 extracted from
- Expression of MdCAS1 and MdCAS2, encoding apple beta-cyanoalanine synthase homologs, is concomitantly induced during ripening and implicates MdCASs in the possible role of the cyanide detoxification in Fuji apple (Malus domestica Borkh.) fruits (2007), Plant Cell Rep., 26, 1321-1331.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethylene | - |
Malus domestica |  |
| additional information | CAS1 and CAS2 mRNAs are coordinately induced during the ripening process of apple fruits, enzyme activity is also enhanced as the fruit ripens, although this increase is not as dramatic as the mRNA induction pattern, genes also activated effectively by mechanical wounding | Malus domestica |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | CAS1 and CAS2 encode Fuji apple beta-CAS homologs, overall nucleotide sequence identity is 89%, whereas coding regions are 93% identical at the nucleotide level and 94% identical at the amino acid level, CAS may play a role in cyanide detoxification in ripening apple fruits | Malus domestica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length MdCAS2 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3) | Malus domestica |
| full-length pMdCAS1 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3) | Malus domestica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40500 | - |
sequence analysis | Malus domestica |
| 40900 | - |
sequence analysis | Malus domestica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | Q1KLZ1 | cv. Fuji | - |
| Malus domestica | Q1KLZ2 | cv. Fuji | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Malus domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Malus domestica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | - |
Malus domestica | H2S + L-3-cyanoalanine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-cyanoalanine synthase | - |
Malus domestica |
| CAS1 | - |
Malus domestica |
| CAS2 | - |
Malus domestica |
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