Any feedback?
Literature summary for 4.4.1.9 extracted from
- Three Arabidopsis genes encoding proteins with differential activities for cysteine synthase and beta-cyanoalanine synthase (2000), Plant Cell Physiol., 41, 465-476.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
KCN | 30°C, pH 10.0 | Arabidopsis thaliana |  |
| 0.04 | - |
Na2S | 30°C, pH 8.0 | Arabidopsis thaliana | |
| 0.14 | - |
L-cysteine | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 8.03 | - |
O-acetyl-L-serine | 30°C, pH 8.0 | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | Arabidopsis thaliana | - |
sulfide + L-3-cyanoalanine | - |
? | |
| O-acetyl-L-serine + sulfide | Arabidopsis thaliana | - |
L-cysteine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9S757 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant AtcysC1 | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | highest mRNA levels in mature rosette leaves | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | - |
Arabidopsis thaliana | sulfide + L-3-cyanoalanine | - |
? | |
| L-cysteine + KCN | - |
Arabidopsis thaliana | L-3-cyanoalanin + K2S | - |
? | |
| L-cysteine + KCN | - |
Arabidopsis thaliana | L-3-cyanoalanine + K2S | - |
? | |
| L-cysteine + NaCN | - |
Arabidopsis thaliana | L-3-cyanoalanin + Na2S | - |
? | |
| O-acetyl-L-serine + sulfide | - |
Arabidopsis thaliana | L-cysteine + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtcysC1 | - |
Arabidopsis thaliana |
| beta-cyanoalanine synthase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
Na2S | 30°C, pH 8.0 | Arabidopsis thaliana | |
| 2 | - |
O-acetyl-L-serine | 30°C, pH 8.0 | Arabidopsis thaliana | |
| 2.17 | - |
KCN | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 2.67 | - |
L-cysteine | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 2.94 | - |
KCN | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 3 | 6 | L-cysteine | 30°C, pH 10.0 | Arabidopsis thaliana | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Arabidopsis thaliana | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
