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Literature summary for 4.4.1.5 extracted from

  • Inoue, Y.; Tran, L.T.; Yoshikawa, K.; Murata, K.; Kimura, A.
    Purification and characterization of glyoxalase I from Hansenula mrakii (1991), J. Ferment. Bioeng., 71, 131-133.
No PubMed abstract available
Search for more literature for 4.4.1.5

Inhibitors

Inhibitors Comment Organism Structure
FeSO4 2.0 mM, 46.5% inhibition Cyberlindnera mrakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.91
-
 methylglyoxal
-
 Cyberlindnera mrakii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Cyberlindnera mrakii

Organism

Organism UniProt Comment Textmining
Cyberlindnera mrakii
-
 IFO 0895
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cyberlindnera mrakii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3570
-
-
 Cyberlindnera mrakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 4,5-dioxovalerate no activity Cyberlindnera mrakii ?
-
 ?
glutathione + methylglyoxal
-
 Cyberlindnera mrakii S-lactoylglutathione
-
 ?
glutathione + phenylglyoxal no activity Cyberlindnera mrakii S-mandeloylglutathione
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Cyberlindnera mrakii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Cyberlindnera mrakii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 40 pH 7.0, 30 min, stable Cyberlindnera mrakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Cyberlindnera mrakii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 4°C, 24 h, stable Cyberlindnera mrakii