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Literature summary for 4.4.1.4 extracted from

  • Yutani, M.; Taniguchi, H.; Borjihan, H.; Ogita, A.; Fujita, K.; Tanaka, T.
    Alliinase from Ensifer adhaerens and its use for generation of fungicidal activity (2011), AMB Express, 1, 2-2.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.4

Application

Application Comment Organism
additional information general properties and evaluation of its application in on-site production of allicin-dependent fungicidal activity Ensifer adhaerens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 (-)-alliin about, below, pH 7.0, 30°C Ensifer adhaerens
2.3
-
 (+/-)-alliin pH 7.0, 30°C Ensifer adhaerens
5.7
-
 (+)-alliin pH 7.0, 30°C Ensifer adhaerens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Ensifer adhaerens
96000
-
 gel filtration Ensifer adhaerens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-alliin Ensifer adhaerens i.e. (-)-S-allyl-L-cysteine sulfoxide, the enzyme is more selectively for (-)-alliin than for (+)-alliin, the enzyme can selectively decompose (-)-alliin, whereas the level of (+)-alliin remains unchanged at least during 30-min incubation allicin + pyruvic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Ensifer adhaerens
-
 isolated from a soil sample
-
Ensifer adhaerens FERM P-19486
-
 isolated from a soil sample
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 300fold to homogeneity by anion exchange, hydrophobic interaction, and aminohexyl affinity chromatography, followed by another different step of anion exchange chromatography and gel filtration Ensifer adhaerens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
 purified enzyme, pH 7.0, 30°C, after gel filtration step Ensifer adhaerens
102.5
-
 purified enzyme, pH 7.0, 30°C, after second anion exchange chromatography step Ensifer adhaerens

Storage Stability

Storage Stability Organism
-20°C - 4°C, purified native enzyme, completely stable for at least 1 week Ensifer adhaerens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-alliin i.e. (+)-S-allyl-L-cysteine sulfoxide, low activity, the enzyme is more selectively for (-)-alliin than for (+)-alliin, a naturally occurring substrate for plant alliinase Ensifer adhaerens allicin + pyruvic acid + NH3
-
 ?
(+/-)-alliin i.e. (+/-)-S-allyl-L-cysteine sulfoxide, the enzyme is more selectively for (-)-alliin than for (+)-alliin, a naturally occurring substrate for plant alliinase Ensifer adhaerens allicin + pyruvic acid + NH3
-
 ?
(+/-)-alliin i.e. (+/-)-S-allyl-L-cysteine sulfoxide, the enzyme is more selectively for (-)-alliin than for (+)-alliin, a naturally occurring substrate for plant alliinase Ensifer adhaerens FERM P-19486 allicin + pyruvic acid + NH3
-
 ?
(+/-)-S-methyl-L-cysteine sulfoxide high activity Ensifer adhaerens ?
-
 ?
(-)-alliin i.e. (-)-S-allyl-L-cysteine sulfoxide, the enzyme is more selectively for (-)-alliin than for (+)-alliin, the enzyme can selectively decompose (-)-alliin, whereas the level of (+)-alliin remains unchanged at least during 30-min incubation Ensifer adhaerens allicin + pyruvic acid + NH3
-
 ?
(-)-alliin i.e. (-)-S-allyl-L-cysteine sulfoxide, best substrate, the enzyme is more selectively for (-)-alliin than for (+)-alliin, a naturally occurring substrate for plant alliinase Ensifer adhaerens allicin + pyruvic acid + NH3
-
 ?
L-cysteine very low activity Ensifer adhaerens ?
-
 ?
L-cysteine very low activity Ensifer adhaerens FERM P-19486 ?
-
 ?
additional information substrate specificity, overview. The C-S lyase activity is also detected with this bacterial enzyme when S-alkyl-L-cysteine is used as a substrate, though such a lyase activity is absolutely absent in alliinase of plant origin, cf. EC 4.4.1.6 Ensifer adhaerens ?
-
 ?
additional information substrate specificity, overview. The C-S lyase activity is also detected with this bacterial enzyme when S-alkyl-L-cysteine is used as a substrate, though such a lyase activity is absolutely absent in alliinase of plant origin, cf. EC 4.4.1.6 Ensifer adhaerens FERM P-19486 ?
-
 ?
S-allyl-L-cysteine high activity Ensifer adhaerens ?
-
 ?
S-ethyl-L-cysteine
-
 Ensifer adhaerens ?
-
 ?
S-ethyl-L-cysteine
-
 Ensifer adhaerens FERM P-19486 ?
-
 ?
S-methyl-L-cysteine low activity Ensifer adhaerens ?
-
 ?
S-methyl-L-cysteine low activity Ensifer adhaerens FERM P-19486 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE Ensifer adhaerens

Synonyms

Synonyms Comment Organism
alliinase
-
 Ensifer adhaerens
P-19486 alliinase
-
 Ensifer adhaerens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Ensifer adhaerens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 purified native enzyme, loss of 42% activity within 30 min Ensifer adhaerens
40 50 purified native enzyme, loss of 50% activity within 30 min, 40% activity remianing after 30 min at 50°C Ensifer adhaerens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Ensifer adhaerens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5 activity range, profile overview Ensifer adhaerens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10.5 purified enzyme, 4°C, completely stable at pH 7.0-9.0, about 50% of maximal activity at pH 5.5 and pH 10.5 Ensifer adhaerens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activity of P-19486 alliinase in the absence of cofactor is 14.3% of that detected in the presence of 0.020 mM cofactor Ensifer adhaerens

General Information

General Information Comment Organism
physiological function the enzyme generates a fungicidal activity against Saccharomyces cerevisiae strain W303-1A in a time- and a dose-dependent fashion using alliin as a stable precursor, overview Ensifer adhaerens