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Literature summary for 4.4.1.4 extracted from
- Immobilization of alliinase and its application: Flow-injection enzymatic analysis for alliin (2009), Afr. J. Biotechnol., 8, 1337-1342.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | Flow-injection enzymatic analytical system for determination of alliin based on the immobilized alliinase and an ammonia gas electrode provids linearity in the 1 × 10-5 to 1 × 10-3 mol/l alliin concentration range and exhibited good repeatability and operational stability | Allium sativum |
General Stability
| General Stability | Organism |
|---|---|
| alliinase is stabilized with 10% glycerol, 0.17 M NaCl and 25 mM pyridoxal-5-phosphate dissolved in phosphate buffer (pH 6.5, 20 mM) | Allium sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-allyl-L-Cys sulfoxide + H2O | Allium sativum | - |
allicin + 2 pyruvate + 2 NH3 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium sativum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| clove | - |
Allium sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-allyl-L-Cys sulfoxide + H2O | - |
Allium sativum | allicin + 2 pyruvate + 2 NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alliinase | - |
Allium sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
immobilized alliinase, the temperature optimum shifts from 30°C to 35°C | Allium sativum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | immobilized alliinase | Allium sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
immobilized alliinase, the optimum pH shifts from 6.0 to 7.0 by the immobilization | Allium sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | immobilized alliinase | Allium sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Allium sativum | |
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