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Literature summary for 4.4.1.4 extracted from

  • Nock, L.P.; Mazelis, M.
    The C-S lyases of higher plants. Direct comparison of the physical properties of homogeneous alliin lyase of garlic (Allium sativum) and onion (Allium cepa) (1987), Plant Physiol., 85, 1079-1083.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, gel filtration on a column equilibrated with 6 M urea Allium sativum
50000
-
 4 * 50000, gel filtration on a column equilibrated with 6 M urea and 0.1% SDS Allium cepa
85000
-
 gel filtration Allium sativum
200000
-
 gel filtration Allium cepa

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-
Allium sativum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Allium cepa
side-chain modification glycoprotein Allium sativum
side-chain modification contains 5.5% carbohydrate, 40% as pentose Allium sativum

Purification (Commentary)

Purification (Comment) Organism
-
 Allium sativum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.2
-
-
 Allium cepa
109
-
-
 Allium sativum

Subunits

Subunits Comment Organism
dimer 2 * 42000, gel filtration on a column equilibrated with 6 M urea Allium sativum
tetramer 4 * 50000, gel filtration on a column equilibrated with 6 M urea and 0.1% SDS Allium cepa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains 1.68 mol of pyridoxal 5'-phosphate per mol of enzyme Allium sativum
pyridoxal 5'-phosphate contains 3.76 mol of pyridoxal 5'-phosphate per mol of enzyme Allium cepa
pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium cepa
pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium sativum