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Literature summary for 4.4.1.4 extracted from

  • Kitamura, N.; Shimomura, N.; Iseki, J.; Honma, M.; Chiba, S.; Tahara, S.; Mizutani, J.
    Cysteine-S-conjugate beta-lyase activity and pyridoxal phosphate binding site of onion alliin lyase (1997), Biosci. Biotechnol. Biochem., 61, 1327-1330.
    View publication on PubMed
Search for more literature for 4.4.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 S-(2-chloro-4-nitrophenyl)-L-Cys alliin lyase II Allium cepa
0.4
-
 S-(2-chloro-6-nitrophenyl)-L-Cys alliin lyase II Allium cepa
0.5
-
 S-(2-chloro-4-nitrophenyl)-L-Cys alliin lyase I Allium cepa
0.6
-
 S-propyl-L-Cys sulfoxide alliin lyase I Allium cepa
0.7
-
 S-(2-chloro-6-nitrophenyl)-L-Cys alliin lyase I Allium cepa
2.1
-
 S-propyl-L-Cys sulfoxide alliin lyase II Allium cepa
7.6
-
 S-phenyl-L-Cys sulfoxide alliin lyase I Allium cepa
9.9
-
 S-phenyl-L-Cys sulfoxide alliin lyase II Allium cepa
10
-
 S-allyl-L-Cys sulfoxide alliin lyase I Allium cepa
11
-
 S-allyl-L-Cys sulfoxide alliin lyase II Allium cepa
16
-
 S-ethyl-L-Cys sulfoxide alliin lyase I Allium cepa
24
-
 S-ethyl-L-Cys sulfoxide alliin lyase II Allium cepa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE Allium cepa

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
alliin lyase I and alliin lyase II Allium cepa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Allium cepa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-ethyl-L-Cys sulfoxide + H2O
-
 Allium cepa ethyl ethanethiosulfinate + 2 pyruvate + 2 NH3
-
 ?
S-(2-chloro-4-nitrophenyl)-L-Cys
-
 Allium cepa ?
-
 ?
S-(2-chloro-6-nitrophenyl)-L-Cys
-
 Allium cepa ?
-
 ?
S-allyl-L-cysteine sulfoxide + H2O
-
 Allium cepa 2-propene-thioic acid + pyruvate + NH4+ + H+
-
 ?
S-phenyl-L-Cys sulfoxide
-
 Allium cepa ?
-
 ?
S-propyl-L-Cys sulfoxide
-
 Allium cepa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Allium cepa

pH Range

pH Minimum pH Maximum Comment Organism
7
-
-
 Allium cepa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Lys285 is the pyridoxal phosphate binding site Allium cepa
pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium cepa