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Literature summary for 4.4.1.31 extracted from

  • Zhao, K.; Wu, D.; Zhou, M.; Zhang, L.; Böhm, S.; Bubenzer, C.; Scheer, H.
    Amino acid residues associated with enzymatic activities of the isomerizing phycoviolobilin-lyase PecE/F (2005), Biochemistry, 44, 8126-8137.
    View publication on PubMed
Search for more literature for 4.4.1.31

Protein Variants

Protein Variants Comment Organism
C122L 4% of wild-type activity Mastigocladus laminosus
C161L 64% of wild-type activity Mastigocladus laminosus
C48Q 78% of wild-type activity Mastigocladus laminosus
C91L 181% of wild-type activity Mastigocladus laminosus
additional information when in subunit PecE the two motifs Y29YAAWWL and D263DLL are deleted, the holoenzyme loses its activity. It is also inactivated upon deletion of a central part, residues R111 to A122 Mastigocladus laminosus
additional information when in subunit PecF the 20 C-terminal and 56 N-terminal amino acids are truncated, the lyase-isomerase activity in combination with subunit PecE decreases to 12% and 15%, respectively, compared to the native enzyme. The catalytic efficiency (kcat/Km) decreases 16fold when the unique four histidine residues in PecF beginning at H53 are deleted. H121 and C122 of PecF are essential for the enzyme activity, they are part of a unique stretch extending from A104 to N125 which is absent in the beta-subunit of related but non-isomerizing lyases Mastigocladus laminosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0059
-
 phycocyanobilin mutant C161L, pH 7.2, 37°C Mastigocladus laminosus
0.01
-
 phycocyanobilin wild-type, pH 7.2, 37°C Mastigocladus laminosus
0.016
-
 phycocyanobilin mutant C48Q, pH 7.2, 37°C Mastigocladus laminosus
0.06
-
 phycocyanobilin mutant C91L, pH 7.2, 37°C Mastigocladus laminosus

Organism

Organism UniProt Comment Textmining
Mastigocladus laminosus P29729 subunit PecE
-
Mastigocladus laminosus P29730 subunit PecF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apo-[phycoerythrocyanin alpha-subunit PecA] + phycocyanobilin subunits PecE and PecF bind phycocyanobilin covalently in substoichiometric amounts Mastigocladus laminosus [phycoerythrocyanin alpha-subunit PecA]-Cys84-phycoviolobilin
-
 ?
apo-[phycoerythrocyanin alpha-subunit PecA] + phycocyanobilin subunits PecE and PecF bind phycocynobilin covalently in substoichiometric amounts Mastigocladus laminosus [phycoerythrocyanin alpha-subunit PecA]-Cys84-phycoviolobilin
-
 ?

Synonyms

Synonyms Comment Organism
pecE
-
 Mastigocladus laminosus
pecF
-
 Mastigocladus laminosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000007
-
 phycocyanobilin mutant C161L, pH 7.2, 37°C Mastigocladus laminosus
0.000018
-
 phycocyanobilin mutant C48Q, pH 7.2, 37°C Mastigocladus laminosus
0.000021
-
 phycocyanobilin wild-type, pH 7.2, 37°C Mastigocladus laminosus
0.00015
-
 phycocyanobilin mutant C91L, pH 7.2, 37°C Mastigocladus laminosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0011
-
 phycocyanobilin mutant C48Q, pH 7.2, 37°C Mastigocladus laminosus
0.0012
-
 phycocyanobilin mutant C161L, pH 7.2, 37°C Mastigocladus laminosus
0.0021
-
 phycocyanobilin wild-type, pH 7.2, 37°C Mastigocladus laminosus
0.0025
-
 phycocyanobilin mutant C91L, pH 7.2, 37°C Mastigocladus laminosus