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Literature summary for 4.4.1.3 extracted from
- The dddP gene of Roseovarius nubinhibens encodes a novel lyase that cleaves dimethylsulfoniopropionate into acrylate plus dimethyl sulfide (2010), Microbiology, 156, 1900-1906.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Roseovarius nubinhibens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D295A | complete loss of activity | Roseovarius nubinhibens |
| D297A | complete loss of activity | Roseovarius nubinhibens |
| D307A | complete loss of activity | Roseovarius nubinhibens |
| E406A | complete loss of activity | Roseovarius nubinhibens |
| E421A | complete loss of activity | Roseovarius nubinhibens |
| H371A | complete loss of activity | Roseovarius nubinhibens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM | Roseovarius nubinhibens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
beta-dimethylsulfoniopropanoate | pH 6.0, 30°C | Roseovarius nubinhibens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels | Roseovarius nubinhibens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49972 | - |
2 * 49972, calculated, 2 * 50000, SDS-PAGE | Roseovarius nubinhibens |
| 50000 | - |
2 * 49972, calculated, 2 * 50000, SDS-PAGE | Roseovarius nubinhibens |
| 95300 | - |
sedimentation equilibrium ultracentrifugation | Roseovarius nubinhibens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Roseovarius nubinhibens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Roseovarius nubinhibens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks | Roseovarius nubinhibens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-dimethylsulfoniopropanoate | - |
Roseovarius nubinhibens | dimethylsulfide + acrylate | - |
? | |
| additional information | enzyme is member of the M24B family, which acts on a non-peptide substrate and has no metal cofactor | Roseovarius nubinhibens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 49972, calculated, 2 * 50000, SDS-PAGE | Roseovarius nubinhibens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dddP | - |
Roseovarius nubinhibens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Roseovarius nubinhibens |
| 55 | 60 | - |
Roseovarius nubinhibens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
rapid loss of activity above | Roseovarius nubinhibens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Roseovarius nubinhibens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | more than 70% of maximum activity | Roseovarius nubinhibens |
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Release 2023.1 (January 2023)
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