Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.5 mM, enhances activity | Treponema denticola |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli LC-67 | Treponema denticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.5 mM | Treponema denticola | |
glycine | - |
Treponema denticola | |
L-alanine | - |
Treponema denticola | |
L-asparagine | less effective inhibitor, Ki-value above 150 mM | Treponema denticola | |
L-methionine | less effective inhibitor, Ki-value above 150 mM | Treponema denticola | |
L-serine | - |
Treponema denticola | |
L-threonine | less effective inhibitor, Ki-value above 150 mM | Treponema denticola | |
L-valine | less effective inhibitor, Ki-value above 150 mM | Treponema denticola | |
additional information | not inhibited by Nalpha-p-tosyl-L-lysine chloromethyl ketone, pronase, proteinase K, CaCl2, MgCl2, ZnCl2, PMSF or benzamidine | Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
L-cysteine | pH 7.4, 22°C | Treponema denticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Treponema denticola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O | Treponema denticola | - |
sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | Treponema denticola LC-67 | - |
sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | Treponema denticola ATCC 35404 | - |
sulfide + NH3 + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
- |
- |
Treponema denticola ATCC 35404 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + H2O | - |
Treponema denticola | NH3 + pyruvate + homocysteine | - |
? | |
cystathionine + H2O | - |
Treponema denticola LC-67 | NH3 + pyruvate + homocysteine | - |
? | |
cystathionine + H2O | - |
Treponema denticola ATCC 35404 | NH3 + pyruvate + homocysteine | - |
? | |
L-cysteine + H2O | - |
Treponema denticola | sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | - |
Treponema denticola LC-67 | sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | - |
Treponema denticola ATCC 35404 | sulfide + NH3 + pyruvate | - |
? | |
S-aminoethyl-L-cysteine + H2O | - |
Treponema denticola | NH3 + pyruvate + cysteamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Treponema denticola |
Synonyms | Comment | Organism |
---|---|---|
cystalysin | - |
Treponema denticola |
L-cysteine desulfhydrase | - |
Treponema denticola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Treponema denticola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
significant decrease in enzymatic activity above 50°C | Treponema denticola |
70 | - |
30 min, complete inactivation | Treponema denticola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
L-cysteine | pH 7.4, 22°C | Treponema denticola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Treponema denticola |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
glycine | pH 7.4, 22°C | Treponema denticola | |
15 | - |
L-serine | pH 7.4, 22°C | Treponema denticola | |
22 | - |
L-alanine | pH 7.4, 22°C | Treponema denticola |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme could function in vivo as a virulence factor | Treponema denticola |