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Literature summary for 4.4.1.23 extracted from

  • Krum, J.G.; Ellsworth, H.; Sargeant, R.R.; Rich, G.; Ensign, S.A.
    Kinetic and microcalorimetric analysis of substrate and cofactor interactions in epoxyalkane:CoM transferase, a zinc-dependent epoxidase (2002), Biochemistry, 41, 5005-5014.
    View publication on PubMed
Search for more literature for 4.4.1.23

Protein Variants

Protein Variants Comment Organism
C220A largely catalytically inactive protein, 0.06% of wild-type activity Xanthobacter autotrophicus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol weak competitive Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 epoxypropane
-
 Xanthobacter autotrophicus
0.034
-
 CoM
-
 Xanthobacter autotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the inactive and Zn-deficient form of enzyme is activated by addition of ZnCl2 or CoCl2 Xanthobacter autotrophicus
Zn Zn plays a key role in activating an organic thiol substrate for nucleophilic attack on an alkyl-donating substrate, removal of Zn results in loss of catalytic activity, the inactive and Zn-deficient form of enzyme is activated by addition of ZnCl2 or CoCl2. Thermodynamic characterization of the interaction of CoM with an enzyme-bound Zn center Xanthobacter autotrophicus
Zn2+ the inactive and Zn-deficient form of enzyme is activated by addition of ZnCl2 or CoCl2 Xanthobacter autotrophicus

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epoxypropane + 3-mercaptopropionate 0.59% of the activity with HS-CoM Xanthobacter autotrophicus 3-[(2-hydroxypropyl)thio]propanoic acid
-
 ?
epoxypropane + HS-CoA 0.51% of the activity with HS-CoM Xanthobacter autotrophicus ?
-
 ?
epoxypropane + HS-CoA 0.11% of the activity with HS-CoM Xanthobacter autotrophicus ?
-
 ?
epoxypropane + HS-CoM
-
 Xanthobacter autotrophicus 2-hydroxypropyl-CoM
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 epoxypropane
-
 Xanthobacter autotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Xanthobacter autotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 85% of maximal activity, pH 9.5: about 90% of maximal activity Xanthobacter autotrophicus