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Literature summary for 4.4.1.21 extracted from

  • Gopishetty, B.; Zhu, J.; Rajan, R.; Sobczak, A.J.; Wnuk, S.F.; Bell, C.E.; Pei, D.
    Probing the catalytic mechanism of S-ribosylhomocysteinase (LuxS) with catalytic intermediates and substrate analogues (2009), J. Am. Chem. Soc., 131, 1243-1250.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.21

Protein Variants

Protein Variants Comment Organism
C83A mutant shows lower activity than the wild type enzyme Vibrio harveyi
E57A mutant shows lower activity than the wild type enzyme Vibrio harveyi
E57D mutant shows lower activity than the wild type enzyme Vibrio harveyi
H11Q mutant shows lower activity than the wild type enzyme Vibrio harveyi
R39M mutant shows lower activity than the wild type enzyme Vibrio harveyi
S6A mutant shows lower activity than the wild type enzyme Vibrio harveyi

Inhibitors

Inhibitors Comment Organism Structure
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
 Bacillus subtilis
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
 Escherichia coli
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
 Vibrio harveyi
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
 Bacillus subtilis
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
 Escherichia coli
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
 Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 S-ribosylhomocysteine wild type enzyme Bacillus subtilis
0.0036
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Bacillus subtilis
0.027
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Escherichia coli
0.027
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Vibrio harveyi
0.037
-
 S-ribosylhomocysteine wild type enzyme Escherichia coli
0.037
-
 S-ribosylhomocysteine wild type enzyme Vibrio harveyi
0.041
-
 S-ribosylhomocysteine mutant enzyme S6A Vibrio harveyi
0.041
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme S6A Vibrio harveyi
0.098
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57A Vibrio harveyi
0.15
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme R39M Vibrio harveyi
0.52
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme H11Q Vibrio harveyi
0.7
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme C83A Vibrio harveyi
0.73
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57D Vibrio harveyi

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site Bacillus subtilis
Fe2+ LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site Escherichia coli
Fe2+ LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid
-
 Bacillus subtilis L-homocysteine + ?
-
 ?
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid
-
 Escherichia coli L-homocysteine + ?
-
 ?
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid
-
 Vibrio harveyi L-homocysteine + ?
-
 ?
S-ribosylhomocysteine
-
 Bacillus subtilis L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?
S-ribosylhomocysteine
-
 Escherichia coli L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?
S-ribosylhomocysteine
-
 Vibrio harveyi L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?

Synonyms

Synonyms Comment Organism
LuxS
-
 Bacillus subtilis
LuxS
-
 Escherichia coli
LuxS
-
 Vibrio harveyi
S-ribosylhomocysteinase
-
 Bacillus subtilis
S-ribosylhomocysteinase
-
 Escherichia coli
S-ribosylhomocysteinase
-
 Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57A Vibrio harveyi
0.016
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme R39M Vibrio harveyi
0.022
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme C83A Vibrio harveyi
0.024
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme H11Q Vibrio harveyi
0.026
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57D Vibrio harveyi
0.027
-
 S-ribosylhomocysteine mutant enzyme S6A Vibrio harveyi
0.03
-
 S-ribosylhomocysteine wild type enzyme Bacillus subtilis
0.038
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Bacillus subtilis
0.14
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme S6A Vibrio harveyi
0.4
-
 S-ribosylhomocysteine wild type enzyme Escherichia coli
0.4
-
 S-ribosylhomocysteine wild type enzyme Vibrio harveyi
0.4
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Escherichia coli
0.64
-
 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Vibrio harveyi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0079
-
 S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Bacillus subtilis
0.0106
-
 S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Bacillus subtilis
0.047
-
 S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Escherichia coli
0.047
-
 S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Vibrio harveyi