Literature summary for 4.4.1.20 extracted from

Zhao, G.; Johnson, M.C.; Schnell, J.R.; Kanaoka, Y.; Haase, W.; Irikura, D.; Lam, B.K.; Schmidt-Krey, I.
Two-dimensional crystallization conditions of human leukotriene C4 synthase requiring adjustment of a particularly large combination of specific parameters (2010), J. Struct. Biol., 169, 450-454.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
large number of parameters are important in obtaining big and well-ordered 2D crystals of LTC4S. Order can be induced by a combination of dividing/selecting fractions during the purification as well as a low lipid-to-protein ratio. To obtain a favorable diameter, salt (optimal: 50 mM), temperature (optimal: 23-24°C), glycerol (optimal: 20%), and initial detergent concentration (optimal: 1%) have to be controlled. Several crystal forms can be grown, namely the plane group symmetries of p2, p3, p312, and two different unit cell sizes of plane group symmetry p321 with unit cell dimensions of a = b = 73.4 A, c = 120 and the second p321 type with larger unit cell size of a = b = 83.0 A, c = 120. Four transmembrane alpha-helices present, to 7.5 A resolution	Homo sapiens

Crystallization (Comment)

Organism

large number of parameters are important in obtaining big and well-ordered 2D crystals of LTC4S. Order can be induced by a combination of dividing/selecting fractions during the purification as well as a low lipid-to-protein ratio. To obtain a favorable diameter, salt (optimal: 50 mM), temperature (optimal: 23-24°C), glycerol (optimal: 20%), and initial detergent concentration (optimal: 1%) have to be controlled. Several crystal forms can be grown, namely the plane group symmetries of p2, p3, p312, and two different unit cell sizes of plane group symmetry p321 with unit cell dimensions of a = b = 73.4 A, c = 120 and the second p321 type with larger unit cell size of a = b = 83.0 A, c = 120. Four transmembrane alpha-helices present, to 7.5 A resolution

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
fractioning	Homo sapiens

Subunits

Subunits	Comment	Organism
trimer	crystallography	Homo sapiens

Synonyms

Synonyms	Comment	Organism
leukotriene C4 synthase	-	Homo sapiens
LTC4S	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)