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Literature summary for 4.4.1.20 extracted from

  • Strid, T.; Svartz, J.; Franck, N.; Hallin, E.; Ingelsson, B.; Soederstroem, M.; Hammarstroem, S.
    Distinct parts of leukotriene C(4) synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein (2009), Biochem. Biophys. Res. Commun., 381, 518-522.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
preparation of pRluc-LTC4S and pGFPLTC4S (full length and truncated forms). Full length and truncated forms of LTC4S excised from the pGFP vector and subcloned in frame with GST in a pGEX vector and transformed into Escherichia coli Y1090. Full length LTC4S subcloned into a pDsRed vector. HEK 293/T cells co-transfected with pRluc-LTC4S and pGFP fusion constructs. COS-7 cells co-transfected with GFP-5-lipoxygenase activating protein and dsRed-LTC4S Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum 5-lipoxygenase activating protein- and LTC4S-fusion proteins co-localize Homo sapiens 5783
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nucleus 5-lipoxygenase activating protein- and LTC4S-fusion proteins co-localize at the nuclear envelope Homo sapiens 5634
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Organism

Organism UniProt Comment Textmining
Homo sapiens Q16873
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-

Synonyms

Synonyms Comment Organism
leukotriene C4 synthase
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Homo sapiens
LTC4S
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Homo sapiens

General Information

General Information Comment Organism
physiological function LTC4S interacts in vitro with both 5-lipoxygenase activating protein and 5-lipoxygenase, these interactions involve distinct parts of LTC4S. 5-lipoxygenase activating protein binds to the N-terminal part/first hydrophobic region of LTC4S, whereas 5-lipoxygenase binds to the second hydrophilic loop of LTC4S. Direct interaction of 5-lipoxygenase and LTC4S in ionophore-stimulated (but not unstimulated) cells Homo sapiens