Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Aspergillus fumigatus |
Application | Comment | Organism |
---|---|---|
medicine | the enzyme displays a strong affinity to homocysteine, followed by methionine and cysteine when compared with non-S amino acids, confirming its potency against homocysteinuria related diseases, and as an anti-cardiovascular agent and a specific biosensor for homocysteinuria | Aspergillus fumigatus |
Protein Variants | Comment | Organism |
---|---|---|
up | induction of the enzyme is mainly sulfur amino-acid-dependent, especially methionine and homocysteine. The induction of the enzyme in response to various amino acids is evaluated, and the results reveal that sulfur-containing amino acids strongly induce Hcy gamma-lyase production by the isolate when compared with other amino acids. The maximum enzyme productivity is detected in the presence of methionine (1.8 U/mg), homocysteine (1.2 U/mg), cysteine (1.23 U/mg), and asparagine (1.2 U/mg). Some enzyme activity is also detected when using various non-sulfur amino acids as substrates, such as glutamine, alanine, and phenylalanine, indicating the multifunctional deaminating catalytic activity of the crude enzyme. The lowest enzyme activity is detected in the presence of tyrosine and lysine | Aspergillus fumigatus |
up | the influence of different carbon sources (0.05-0.2%) on the enzyme is assessed when using 100 mM L-methionine as the substrate. From the data, the highest enzyme yield is obtained with 0.15% sucrose (2.54 U/mg), followed by glucose (2.18 U/mg), representing about 1.42- and 1.2-fold increments, respectively, over the control (1.8 U/mg). The enzyme productivity increases about 12.7fold when using 0.15% sucrose over a carbon-free medium | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10 mM, 86% loss of activity | Aspergillus fumigatus | |
Hg2+ | 10 mM, 95% loss of activity | Aspergillus fumigatus | |
hydroxylamine | 10 mM, 89% loss of activity | Aspergillus fumigatus | |
KCl | 10 mM, 11% loss of activity | Aspergillus fumigatus | |
additional information | no inhibition by EDTA | Aspergillus fumigatus | |
SDS | 10 mM, 78% loss of activity | Aspergillus fumigatus | |
Tween 80 | 1 mM, about 40% loss of activity | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.46 | - |
L-homocysteine | pH 7.0, 35°C | Aspergillus fumigatus | |
4.1 | - |
L-methionine | pH 7.0, 35°C | Aspergillus fumigatus | |
4.9 | - |
L-cysteine | pH 7.0, 35°C | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus fumigatus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homocysteine + H2O | Aspergillus fumigatus | - |
hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? | |
L-homocysteine + H2O | Aspergillus fumigatus ASH JX006238 | - |
hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Aspergillus fumigatus ASH JX006238 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus fumigatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.48 | - |
pH 7.0, 35°C, solid state fermentation, purified enzyme | Aspergillus fumigatus |
16.6 | - |
pH 7.0, 35°C, submerged fermentation, purified enzyme | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O | 26.3% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus | ? | - |
? | |
glycine + H2O | 26.3% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus ASH JX006238 | ? | - |
? | |
L-alanine + H2O | 32.1% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus | ? | - |
? | |
L-alanine + H2O | 32.1% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus ASH JX006238 | ? | - |
? | |
L-asparagine + H2O | 33.3% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus | ? | - |
? | |
L-asparagine + H2O | 33.3% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus ASH JX006238 | ? | - |
? | |
L-cysteine + H2O | 43.3% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus | 2-oxopropanoate + NH3 + ? | - |
? | |
L-cystine + H2O | 16.2% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus | ? | - |
? | |
L-cystine + H2O | 16.2% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus ASH JX006238 | ? | - |
? | |
L-homocysteine + H2O | - |
Aspergillus fumigatus | hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? | |
L-homocysteine + H2O | - |
Aspergillus fumigatus ASH JX006238 | hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? | |
L-lysine + H2O | 27.1% of the oxo-acid release activity compared to L-homocysteine as substrate | Aspergillus fumigatus | ? | - |
? | |
L-methionine + H2O | 90% of the oxo-acid release compared to L-homocysteine as substrate | Aspergillus fumigatus | 2-oxobutanoate + NH3 + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
Hyc gamma-lyase | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | - |
Aspergillus fumigatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
T1/2: 18.1 h, at 30°C | Aspergillus fumigatus |
35 | - |
T1/2: 9.9 h, at 30°C | Aspergillus fumigatus |
40 | - |
stable below, T1/2: 5.9 h, at 30°C | Aspergillus fumigatus |
50 | - |
T1/2: 3.3 h, at 30°C | Aspergillus fumigatus |
60 | - |
T1/2: 1.9 h, at 30°C | Aspergillus fumigatus |
70 | - |
Tm-value is 70.1°C. Retention of about 50% of its initial activity when heated for 60 min at 70.1°C | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
L-cysteine | pH 7.0, 35°C | Aspergillus fumigatus | |
0.00097 | - |
L-methionine | pH 7.0, 35°C | Aspergillus fumigatus | |
0.00139 | - |
L-homocysteine | pH 7.0, 35°C | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Aspergillus fumigatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
4°C, 2 h, 50% reduction in enzyme activity | Aspergillus fumigatus |
6.5 | 7.8 | maximal structural and catalytic stability within the pH-range 6.5-7.8 | Aspergillus fumigatus |
10.4 | - |
4°C, 2 h, 20% reduction in enzyme activity | Aspergillus fumigatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus fumigatus | isoelectric focusing, detected by pH precipitation with no glycosyl residues | - |
5 |