Cloned (Comment) | Organism |
---|---|
sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in dimeric and tetrameric forms, sitting drop vapour diffusion method, 100 nl of 17 mg/ml protein solution with 100 nl of well solution 1 containing 100 mM HEPES, pH 6.7, and 10% PEG 6000, or well solution 2 containing 50 mM HEPES, pH 8.1, 200 mM ammonium nitrate and 25% PEG 3350, 20°C, 3 days or 7 days, respectively, X-ray diffraction structure determination and analysis of the two crystals forms at 2.1 A and 1.8 A resolution, respectively, modeling of dimeric enzyme complexed with pyridoxal 5'-phosphate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D146K | site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity | Homo sapiens |
D146K/H389T | site-directed mutagenesis,the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity to a higher extent | Homo sapiens |
H389T | site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity | Homo sapiens |
V256S | site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | Homo sapiens | - |
selenide + L-alanine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96I15 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage through TEV protease, another step of nickel affinity chromatography, and dialysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | - |
Homo sapiens | selenide + L-alanine + acceptor | - |
? | |
additional information | the enzyme is specific for L-selenocysteine versus L-cysteine, substrate specificity for selenium and sulfur is determined by a single residue, Asp246, in human selenocysteine lyase | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCL | - |
Homo sapiens |
Sec lyase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified enzyme, pH 8.5, 2 min, inactivation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme adopts the canonical fold type I structure of pyridoxal 5'-phosphate enzymes with an active site cysteine, C388 | Homo sapiens |