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Literature summary for 4.4.1.16 extracted from

  • Johansson, A.L.; Collins, R.; Arner, E.S.; Brzezinski, P.; Hoegbom, M.
    Biochemical discrimination between selenium and sulfur 2: mechanistic investigation of the selenium specificity of human selenocysteine lyase (2012), PLoS ONE, 7, e30528.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.16

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
D146K site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity Homo sapiens
D146K/H389T site-directed mutagenesis,the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity to a higher extent Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-selenocysteine + reduced acceptor Homo sapiens
-
 selenide + L-alanine + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96I15
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage through TEV protease, another step of nickel affinity chromatography, and dialysis Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor reaction mechanism and mechanism of substrate recognition, model, overview. A pyridoxal 5'-phosphate-substrate intermediate is attacked by a deprotonated active site Cys, producing the protein-bound persulfide or sulfoselenide, formation of the external Cys-aldimine intermediate. Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-selenocysteine + reduced acceptor
-
 Homo sapiens selenide + L-alanine + acceptor
-
 ?
additional information the enzyme is specific for L-selenocysteine versus L-cysteine, substrate specificity for selenium and sulfur is determined by a single residue, Asp246, in human selenocysteine lyase, chemical basis for and mechanism of the enzyme's substrate discrimination of wild-type enzyme and mutant D146K/H389T, overview. The wild-type reaction with Cys is a single-turnover reaction, producing a dead-end protein adduct Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
SCL
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens