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Literature summary for 4.4.1.16 extracted from

  • Mihara, H.; Kurihara, T.; Watanabe, T.; Yoshimura, T.; Esaki, N.
    cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis (2000), J. Biol. Chem., 275, 6195-6200.
    View publication on PubMed
Search for more literature for 4.4.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.6
-
 L-cysteine sulfinate pH 9.0, 37°C Mus musculus
9.9
-
 L-selenocysteine pH 9.0, 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Mus musculus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 SDS-PAGE Mus musculus
47200
-
 calculated from amino acid sequence Mus musculus
105000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-selenocysteine + reduced acceptor Sus scrofa probably functions as selenide delivery system selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor Mus musculus probably functions as selenide delivery system selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor Sus scrofa most likely metabolizes small amounts of selenocysteine in tissue selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor Mus musculus most likely metabolizes small amounts of selenocysteine in tissue selenide + L-alanine + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9JLI6
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa
overexpressed protein Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
heart
-
 Sus scrofa
-
heart
-
 Mus musculus
-
kidney
-
 Sus scrofa
-
kidney
-
 Mus musculus
-
liver
-
 Sus scrofa
-
liver
-
 Mus musculus
-
lung
-
 Mus musculus
-
spleen
-
 Mus musculus
-
stomach
-
 Mus musculus
-
testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29
-
 purified enzyme, pH 7.4 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine sulfinic acid + reduced acceptor less than 10% of activity Mus musculus SO2 + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor
-
 Sus scrofa selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor
-
 Mus musculus selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor probably functions as selenide delivery system Sus scrofa selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor probably functions as selenide delivery system Mus musculus selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor most likely metabolizes small amounts of selenocysteine in tissue Sus scrofa selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor most likely metabolizes small amounts of selenocysteine in tissue Mus musculus selenide + L-alanine + acceptor
-
 ?
additional information
-
 Sus scrofa ?
-
 ?
additional information traces of activity with L-cysteine Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Sus scrofa
dimer 2 * 47000, SDS-PAGE Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 L-cysteine sulfinate pH 9.0, 37°C Mus musculus
46
-
 L-selenocysteine pH 9.0, 37°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Sus scrofa
9
-
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Sus scrofa
pyridoxal 5'-phosphate required Mus musculus