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Literature summary for 4.4.1.16 extracted from

  • Esaki, N.; Soda, K.
    Selenocysteine beta-lyase from Citrobacter freundii (1987), Methods Enzymol., 143, 493-496.
No PubMed abstract available

General Stability

General Stability Organism
labile during purification, 0.1% 2-mercaptoethanol, 1 mM dithiothreitol or 1 M sucrose stabilizes activity Citrobacter freundii

Inhibitors

Inhibitors Comment Organism Structure
beta-chloro-L-alanine
-
Citrobacter freundii
Hg2+
-
Citrobacter freundii
iodoacetamide 1 mM, complete inactivation within 3 h Citrobacter freundii
iodoacetic acid 1 mM, complete inactivation within 3 h Citrobacter freundii
L-cysteine
-
Citrobacter freundii
L-cysteine methyl ester
-
Citrobacter freundii
N-ethylmaleimide 1 mM, complete inactivation within 3 h Citrobacter freundii
NaBH4
-
Citrobacter freundii
Ni2+
-
Citrobacter freundii
Pb2+
-
Citrobacter freundii
Zn2+
-
Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.95
-
L-selenocysteine
-
Citrobacter freundii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
HPLC gel filtration Citrobacter freundii
64000
-
SDS-PAGE Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-selenocysteine + reduced acceptor Citrobacter freundii
-
selenide + L-alanine + acceptor
-
?
L-selenocysteine + reduced acceptor Citrobacter freundii IRC 0070
-
selenide + L-alanine + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-
Citrobacter freundii IRC 0070
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Citrobacter freundii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.47
-
purified enzyme Citrobacter freundii

Storage Stability

Storage Stability Organism
-20°C, 1 month, 60% loss of activity Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-alanine alpha,beta elimination, irreversible inactivation of enzyme during the course of reaction Citrobacter freundii NH3 + pyruvate + chloride
-
?
beta-chloro-L-alanine alpha,beta elimination, irreversible inactivation of enzyme during the course of reaction Citrobacter freundii IRC 0070 NH3 + pyruvate + chloride
-
?
L-selenocysteine + reduced acceptor
-
Citrobacter freundii selenide + L-alanine + acceptor
-
?
L-selenocysteine + reduced acceptor
-
Citrobacter freundii IRC 0070 selenide + L-alanine + acceptor
-
?
additional information no activity with L-cysteine Citrobacter freundii ?
-
?
additional information no activity with L-cysteine Citrobacter freundii IRC 0070 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Citrobacter freundii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Citrobacter freundii
pyridoxal 5'-phosphate 1 mol bound per mol enzyme (subunit) Citrobacter freundii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
L-cysteine methyl ester
-
Citrobacter freundii
2.5
-
beta-chloro-L-alanine
-
Citrobacter freundii