Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-Keto acids | restore activity during reaction | Citrobacter freundii |
General Stability | Organism |
---|---|
labile during purification, 0.1% 2-mercaptoethanol, 1 mM dithiothreitol or 1 M sucrose stabilizes activity | Citrobacter freundii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-chloro-L-alanine | irreversible | Citrobacter freundii | |
Hg2+ | - |
Citrobacter freundii | |
iodoacetamide | 1 mM, complete inactivation within 3 h | Citrobacter freundii | |
iodoacetic acid | 1 mM, complete inactivation within 3 h | Citrobacter freundii | |
L-cysteine | competitive | Citrobacter freundii | |
L-cysteine methyl ester | competitive | Citrobacter freundii | |
N-ethylmaleimide | 1 mM, complete inactivation within 3 h | Citrobacter freundii | |
NaBH4 | irreversible | Citrobacter freundii | |
Ni2+ | - |
Citrobacter freundii | |
Pb2+ | - |
Citrobacter freundii | |
Zn2+ | - |
Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
L-selenocysteine | pH 7.2 | Citrobacter freundii | |
3.1 | - |
beta-chloro-L-alanine | pH 7.2 | Citrobacter freundii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Citrobacter freundii | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
HPLC gel filtration | Citrobacter freundii |
64000 | - |
SDS-PAGE | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | Citrobacter freundii | - |
selenide + L-alanine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Citrobacter freundii |
Storage Stability | Organism |
---|---|
-20°C, 1 month, 60% loss of activity | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloro-L-alanine | alpha,beta elimination, irreversible inactivation of enzyme during the course of reaction | Citrobacter freundii | NH3 + pyruvate + chloride | - |
? | |
L-selenocysteine + reduced acceptor | - |
Citrobacter freundii | selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | strictly specific, no activity with D-isomer, enzyme is inativated during course of reaction, inactivation can be prevented by addition of alpha-keto acids | Citrobacter freundii | selenide + L-alanine + acceptor | formation of H2Se by nonenzymatic reduction of elemental selenium in presence of dithiothreitol | ? | |
L-selenocysteine + reduced acceptor | enzyme bound pyridoxal 5'-phosphate serves as acceptor | Citrobacter freundii | selenide + L-alanine + acceptor | formation of H2Se by nonenzymatic reduction of elemental selenium in presence of dithiothreitol | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, SDS-PAGE | Citrobacter freundii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Citrobacter freundii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Citrobacter freundii | |
pyridoxal 5'-phosphate | 1 mol bound per mol enzyme (subunit) | Citrobacter freundii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
L-cysteine methyl ester | pH 7.2 | Citrobacter freundii | |
0.65 | - |
L-cysteine | pH 7.2 | Citrobacter freundii | |
2.5 | - |
beta-chloro-L-alanine | pH 7.2 | Citrobacter freundii |