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Literature summary for 4.4.1.16 extracted from

  • Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N.
    Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions (2000), J. Biochem., 127, 559-567.
    View publication on PubMed
Search for more literature for 4.4.1.16

Activating Compound

Activating Compound Comment Organism Structure
pyruvate
-
 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C364A increased activity towards L-selenocysteine and L-cysteine sulfinate, activity toward L-cysteine was completely abolished Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
 L-selenocysteine in the presence of pyruvate, pH 7.5 Escherichia coli
30
-
 L-cysteine sulfinate in the presence of pyruvate, pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-selenocysteine + reduced acceptor Escherichia coli
-
 selenide + L-alanine + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpressed protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine sulfinic acid + reduced acceptor
-
 Escherichia coli SO2 + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor
-
 Escherichia coli selenide + L-alanine + acceptor
-
 ?
L-selenocysteine + reduced acceptor enzyme bound pyridoxal 5'-phosphate serves as acceptor Escherichia coli selenide + L-alanine + acceptor
-
 ?
additional information traces of activity with L-cysteine Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
More two other proteins (CDS and IscS) with activity towards L-selenocysteine identified that are much less specific Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Escherichia coli