Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cucurbita pepo |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
S-adenosyl-L-methionine | refolded enzyme | Cucurbita pepo |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
refolded enzyme, gel filtration | Cucurbita pepo |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | Cucurbita pepo | biosynthesis of ethylene: plant hormone | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita pepo | P23279 | zucchini | - |
Purification (Comment) | Organism |
---|---|
inclusion body purified | Cucurbita pepo |
Renatured (Comment) | Organism |
---|---|
refolding by a combination of dialysis and dilution in 100mM MOPS, pH 8, 30 mM Chaps and 5 mM GSH | Cucurbita pepo |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
refolded enzyme | Cucurbita pepo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | biosynthesis of ethylene: plant hormone | Cucurbita pepo | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? |