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Literature summary for 4.4.1.13 extracted from

  • Kato, S.; Oikawa, T.
    A novel bifunctional amino acid racemase with multiple substrate specificity, malY from Lactobacillus sakei LT-13 Genome-based identification and enzymological characterization (2018), Front. Microbiol., 9, 403 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
gene malY, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Latilactobacillus sakei

Protein Variants

Protein Variants Comment Organism
K233A site-directed mutagenesis, the mutant is only active with D-Ser, but inactive with L- or D-Cys, and L-Ser, in contrast to the wild-type enzyme Latilactobacillus sakei
Y123A site-directed mutagenesis, the mutant shows highly reduced activity with L-Cys compared to the wild-type enzyme, and is active with L-Ser in contrast to wild-type Latilactobacillus sakei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.5
-
 L-cysteine pH 10.0, 35°C, recombinant His-tagged enzyme Latilactobacillus sakei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
281000
-
 recombinant His6-tagged enzyme, gel filtration Latilactobacillus sakei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cystathionine + H2O Latilactobacillus sakei overall reaction L-homocysteine + pyruvate + NH3
-
 ?
L-cysteine + H2O Latilactobacillus sakei
-
 ? + NH3
-
 ?
L-cystine + H2O Latilactobacillus sakei
-
 ? + NH3
-
 ?
additional information Latilactobacillus sakei Ls-MalY is a bifunctional amino acid racemase with multiple substrate specificity, both the amino acid racemase and beta-lyase reactions of Ls-MalY are catalyzed at the same active site ?
-
 ?

Organism

Organism UniProt Comment Textmining
Latilactobacillus sakei A0A223K4L6
-
-
Latilactobacillus sakei LT-13 A0A223K4L6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, to homogeneity Latilactobacillus sakei

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain LK-145 is isolated from a Japanese sake seller as a high D-amino acid producer, and strain LT-13 is isolated from Moto, a starter of sake, as a low D-amino acid producer, using a medium of amylase digested rice as a carbon source Latilactobacillus sakei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O overall reaction Latilactobacillus sakei L-homocysteine + pyruvate + NH3
-
 ?
L-cysteine + H2O
-
 Latilactobacillus sakei ? + NH3
-
 ?
L-cystine + H2O
-
 Latilactobacillus sakei ? + NH3
-
 ?
additional information Ls-MalY is a bifunctional amino acid racemase with multiple substrate specificity, both the amino acid racemase and beta-lyase reactions of Ls-MalY are catalyzed at the same active site Latilactobacillus sakei ?
-
 ?
additional information enzyme Ls-MalY shows cystathionine beta-lyase and amino acid racemase activity (EC 5.1.1.10) with various amino acids, such as Ala, Arg, Asn, Glu, Gln, His, Leu, Lys, Met, Ser, Thr, Trp, and Val. The beta-lyase shows activity toward L-Cys, but not toward D-Cys, L-Ser, and D-Ser, substrate specificity, overview. Ls-MalY also shows beta-lyase activity with L-cystine and L-cystathionine. The epsilon-amino group of Lys233 in the primary structure of Ls-MalY likely bound to pyridoxal 5'-phosphate, and Lys233 is an essential residue for Ls-MalY to catalyze both the amino acid racemase and beta-lyase reactions. Tyr123 is a catalytic residue in the amino acid racemase reaction but strongly affects beta-lyase activity Latilactobacillus sakei ?
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 46000, recombinant His6-tagged enzyme, SDS-PAGE Latilactobacillus sakei
More structural modeling of Ls-MalY, structure comparisons, overview Latilactobacillus sakei

Synonyms

Synonyms Comment Organism
beta-lyase
-
 Latilactobacillus sakei
LACBS_00576 locus name Latilactobacillus sakei
MalY
-
 Latilactobacillus sakei
patB
-
 Latilactobacillus sakei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Latilactobacillus sakei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 activity range, profile overview Latilactobacillus sakei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
930
-
 L-cysteine pH 10.0, 35°C, recombinant His-tagged enzyme Latilactobacillus sakei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Latilactobacillus sakei

pH Range

pH Minimum pH Maximum Comment Organism
7.5 11.5 activity range, profile overview Latilactobacillus sakei

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Latilactobacillus sakei

General Information

General Information Comment Organism
additional information structural modeling of Ls-MalY, structure comparisons, overview Latilactobacillus sakei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
80.87
-
 L-cysteine pH 10.0, 35°C, recombinant His-tagged enzyme Latilactobacillus sakei