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Literature summary for 4.4.1.11 extracted from
- Methionine depletion with recombinant methioninase: in vitro and in vivo efficacy against neuroblastoma and its synergism with chemotherapeutic drugs (2009), Int. J. Cancer, 124, 1700-1706.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | recombinant METase alone inhibits the proliferation of 15/15 NB cell lines in vitro in a dose-dependent manner | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| QAE-Sepharose column chromatography | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM potassium phosphate buffer (pH 7.4) with 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA (pH 8.0), 0.01% (v/v) beta-mercaptoethanol containing 0.3 M NaCl, 2 months, enzyme remains stable | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-methionine gamma-lyase | - |
Pseudomonas putida |
| METase | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pseudomonas putida | |
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