Any feedback?
Literature summary for 4.4.1.11 extracted from
- L-methionine gamma-lyase from Pseudomonas putida and Aeromonas (1987), Methods Enzymol., 143, 459-465.
General Stability
| General Stability | Organism |
|---|---|
| significantly inactivated in dilute buffer | Pseudomonas putida |
| significantly inactivated in dilute buffer | Aeromonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| carbonyl reagents | - |
Aeromonas sp. | |
| carbonyl reagents | - |
Pseudomonas putida | |
| hydroxylamine | - |
Aeromonas sp. |  |
| hydroxylamine | - |
Pseudomonas putida | |
| L-Cycloserine | - |
Aeromonas sp. | |
| L-Cycloserine | - |
Pseudomonas putida | |
| N-ethylmaleimide | - |
Aeromonas sp. | |
| N-ethylmaleimide | - |
Pseudomonas putida | |
| NaBH4 | - |
Aeromonas sp. | |
| NaBH4 | - |
Pseudomonas putida | |
| Penicillamine | - |
Aeromonas sp. | |
| Penicillamine | - |
Pseudomonas putida | |
| sulfhydryl inhibitors | - |
Aeromonas sp. | |
| sulfhydryl inhibitors | - |
Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas sp. | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| NaBH4, irreversible inactivation | Pseudomonas putida |
| NaBH4, irreversible inactivation | Aeromonas sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aeromonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
| additional information | - |
- |
Aeromonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.2, potassium phosphate buffer, 0.1 M, several months | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Pseudomonas putida | ? | - |
? | |
| additional information | - |
Aeromonas sp. | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to 30 min | Pseudomonas putida |
| 50 | - |
stable up to 30 min | Aeromonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | stable, at least 3 h | Pseudomonas putida |
| 6 | 9 | stable, at least 3 h | Aeromonas sp. |
| 6 | 9 | at 0°C | Pseudomonas putida |
| 6 | 9 | at 0°C | Aeromonas sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
