Protein Variants | Comment | Organism |
---|---|---|
E333A | site-directed mutagenesis of the active-site residue, pH optimum 7.8-8.6, the mutant shows increased KM for L-cystathionine up to 17fold, and 2.5fold for O-acetyl-L-serine | Saccharomyces cerevisiae |
E333D | site-directed mutagenesis of the active-site residue, pH optimum 7.2-8.0, the mutant shows increased KM for L-cystathionine up to 17fold, and 2.5fold for O-acetyl-L-serine | Saccharomyces cerevisiae |
E333Q | site-directed mutagenesis of the active-site residue, pH optimum 7.6-8.4, the mutant shows increased KM for L-cystathionine up to 17fold, and 2.5fold for O-acetyl-L-serine | Saccharomyces cerevisiae |
E48A | site-directed mutagenesis of the active-site residue, pH optimum 7.6-8.4 | Saccharomyces cerevisiae |
E48A/E333A | site-directed mutagenesis of the active-site residues, pH optimum 8.4-9.2 | Saccharomyces cerevisiae |
E48D | site-directed mutagenesis of the active-site residue, pH optimum 7.07.8 | Saccharomyces cerevisiae |
E48D/E333D | site-directed mutagenesis of the active-site residues, pH optimum 7.6-8.4 | Saccharomyces cerevisiae |
E48Q | site-directed mutagenesis of the active-site residue, pH optimum 7.4-8.2 | Saccharomyces cerevisiae |
E48Q/E333Q | site-directed mutagenesis of the active-site residues, pH optimum 7.9-8.7 | Saccharomyces cerevisiae |
additional information | pH optima of the mutant enzymes vary from wild-type enzyme, overview | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Saccharomyces cerevisiae | |
0.09 | - |
L-cysteine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
0.11 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.18 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
0.4 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
0.4 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
0.5 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
0.5 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.7 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.7 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.71 | - |
L-cystathionine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
0.76 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.87 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
0.9 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
0.9 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
1.2 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
1.4 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
1.5 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
1.5 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
1.8 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
2.2 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
2.5 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
2.9 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
4.4 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
5.3 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
12 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
12 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
13 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | Saccharomyces cerevisiae | - |
L-cysteine + 2-oxobutanoate + NH3 | - |
r | |
L-cysteine + H2O | Saccharomyces cerevisiae | - |
H2S + pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Saccharomyces cerevisiae | L-cysteine + 2-oxobutanoate + NH3 | - |
r | |
L-cysteine + H2O | - |
Saccharomyces cerevisiae | H2S + pyruvate + NH3 | - |
? | |
additional information | Glu333 of cystathionine gamma-lyase acts as a determinant of specificity, it interacts with the distal amine moiety of L-cystathionine, which is not present in the alternative substrate O-acetyl-L-serine. Catalytic efficiency of the yeast enzyme for alpha,gamma-elimination of O-succinyl-L-homoserine, which possesses a distal carboxylate, but lacks an amino group, is 300fold lower than that of the physiological L-cystathionine substrate and 260fold higher than that of L-Hcys, which lacks both distal polar moieties. Proposed polar contacts of the yCGL active site that interact with or are influenced by E48 and E333 overview | Saccharomyces cerevisiae | ? | - |
? | |
O-acetyl-L-serine + H2O | - |
Saccharomyces cerevisiae | acetate + pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cgl | - |
Saccharomyces cerevisiae |
yCGL | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.0045 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
0.0047 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
0.0053 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
0.0058 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
0.0092 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
0.0097 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
0.0102 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.0124 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
0.0142 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.029 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.09 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.11 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
0.15 | - |
L-cysteine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
0.16 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
0.18 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
0.24 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
0.32 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
0.48 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.5 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.54 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.78 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
0.98 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
1.29 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
1.3 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
1.4 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
1.51 | - |
L-cystathionine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | wild-type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | cystathionine gamma-lyase catalyzes the hydrolysis of L-cystathionine in the second step of the reverse transsulfuration pathway, which converts L-homocysteine to L-Cys | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
0.0019 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.0026 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
0.0028 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
0.0037 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
0.0038 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant wild-type enzyme and mutant E48D | Saccharomyces cerevisiae | |
0.005 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
0.005 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.006 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
0.0088 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.01 | - |
O-acetyl-L-serine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.0169 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
0.036 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.06 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48D/E333D | Saccharomyces cerevisiae | |
0.065 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
0.082 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
0.13 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333D | Saccharomyces cerevisiae | |
0.21 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48A/E333A | Saccharomyces cerevisiae | |
0.31 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48Q/E333Q | Saccharomyces cerevisiae | |
0.49 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333Q | Saccharomyces cerevisiae | |
0.71 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.72 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
0.8 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48A | Saccharomyces cerevisiae | |
0.81 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E333A | Saccharomyces cerevisiae | |
1.02 | - |
L-cysteine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
1.4 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48Q | Saccharomyces cerevisiae | |
1.59 | - |
L-cystathionine | pH 7.2, 25°C, recombinant mutant E48D | Saccharomyces cerevisiae | |
1.7 | - |
L-cysteine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
2.1 | - |
L-cystathionine | pH 7.2, 25°C, recombinant wild-type enzyme | Saccharomyces cerevisiae |