Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces cremeus |
Crystallization (Comment) | Organism |
---|---|
free enzyme and enzyme in complex with fumarate, using 0.1 M Tris/HCl pH 8.2-8.5 and 1.8-2.0 M ammonium sulfate | Streptomyces cremeus |
Protein Variants | Comment | Organism |
---|---|---|
D109N | the mutant loses the activity to produce nitrite almost completely | Streptomyces cremeus |
D109V | the mutant loses the activity to produce nitrite almost completely | Streptomyces cremeus |
H237A | the mutant loses the activity to produce nitrite completely | Streptomyces cremeus |
K292A | the mutant loses the activity to produce nitrite completely | Streptomyces cremeus |
N294A | the mutant loses the activity to produce nitrite almost completely | Streptomyces cremeus |
N77A | the mutation slightly weakens the nitrite production compared to the wild type enzyme | Streptomyces cremeus |
R325A | the mutant loses the activity to produce nitrite completely | Streptomyces cremeus |
S286A | the mutant loses the activity to produce nitrite almost completely | Streptomyces cremeus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
203000 | - |
calculated from amino acid sequence | Streptomyces cremeus |
228500 | - |
gel filtration with inline multiangle light scattering | Streptomyces cremeus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrobutanedioate | Streptomyces cremeus | - |
fumarate + nitrite | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cremeus | A0A0K2JL82 | - |
- |
Purification (Comment) | Organism |
---|---|
His60 Ni Superflow resin column chromatography and Resource Q column chromatography | Streptomyces cremeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrobutanedioate | - |
Streptomyces cremeus | fumarate + nitrite | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Streptomyces cremeus |
Synonyms | Comment | Organism |
---|---|---|
creD | - |
Streptomyces cremeus |