Cloned (Comment) | Organism |
---|---|
STR gene, expresssion of wild-type enzyme and mutant constructs in Escherichia coli strain BL21(DE3)pLysS | Rauvolfia serpentina |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a STR tandem gene sequence, that serves as template for the preparation of circular permutated STR variants is assembled in pET17b using a truncated version of the STR gene, the N-terminal 28 amino acid residues in STR which constitutes the native signal sequence, as well as the unstructured 11 amino acids at the C-terminus are deleted, leaving a 915-nucleotide truncated version of STR, several truncated variants all show reduced activity compared to the wild-type enzyme, overview | Rauvolfia serpentina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | Rauvolfia serpentina | the enzyme is as amine lyases and catalyzes a PictetSpengler-type condensation between tryptamine and the aldehyde secologanin which leads to the formation of (S)-strictosidine | 3-alpha(S)-strictosidine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | - |
Rauvolfia serpentina | 3-alpha(S)-strictosidine + H2O | - |
? | |
tryptamine + secologanin | the enzyme is as amine lyases and catalyzes a PictetSpengler-type condensation between tryptamine and the aldehyde secologanin which leads to the formation of (S)-strictosidine | Rauvolfia serpentina | 3-alpha(S)-strictosidine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is a six bladed beta-propeller protein | Rauvolfia serpentina |
Synonyms | Comment | Organism |
---|---|---|
str | - |
Rauvolfia serpentina |
General Information | Comment | Organism |
---|---|---|
evolution | strictosidine synthases belong to the beta-propeller fold family characterized by an all-beta architecture with four to eight blades, each consisting of four antiparallel beta-sheets, arranged around a pseudo symmetry axis | Rauvolfia serpentina |
additional information | the six bladed beta-propeller protein has two regions which cover the enzyme active site. The observed activity changes suggest important roles of both regions in protein folding, stability and catalysis | Rauvolfia serpentina |
physiological function | strictosidine synthases catalyze the formation of strictosidine, a key intermediate in the biosynthesis of a large variety of monoterpenoid indole alkaloids | Rauvolfia serpentina |