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Literature summary for 4.3.3.2 extracted from
- Rapid identification of enzyme variants for reengineered alkaloid biosynthesis in periwinkle (2007), Chem. Biol., 14, 888-897.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | modulating the substrate specificity of strictosidine synthase is a critical step toward indole alkaloid pathway engineering to yield nonnatural alkaloids | Catharanthus roseus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned into the pGEM-T Easy vector, excised and ligated into pGAL-MF to obtain pSTSMF-FLAG for expression in Saccharomyces cerevisiae, and into pET28a+ to obtain pSTSMF-HIS for expression in Escherichia coli | Catharanthus roseus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | in a rational redesign approach all 20 naturally occuring amino acids at selected residues in the tryptamine-binding pocket are probed | Catharanthus roseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
tryptamine | wild-type strictosidine synthase expressed in yeast | Catharanthus roseus |  |
| 0.37 | - |
5-methyltryptamine | mutant V214M | Catharanthus roseus | |
| 0.42 | - |
tryptamine | mutant V214M | Catharanthus roseus | |
| 0.44 | - |
tryptamine | mutant E315A | Catharanthus roseus | |
| 0.62 | - |
tryptamine | mutant F232L | Catharanthus roseus | |
| 0.94 | - |
(2'R)-tryptophanol | mutant F232L | Catharanthus roseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
determined by SDS-PAGE | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-alpha(S)-strictosidine + H2O | Catharanthus roseus | - |
tryptamine + secologanin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R)-tryptophanol + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| (2S)-tryptophanol + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| 3-alpha(S)-strictosidine + H2O | - |
Catharanthus roseus | tryptamine + secologanin | - |
? | |
| 5-bromotryptamine + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| 5-chlorotryptamine + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| 5-methyltryptamine + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| D-tryptophan + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| D-tryptophan methyl ester + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
| L-tryptophan + H2O | substrate for discovery of active mutants | Catharanthus roseus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| strictosidine synthase | - |
Catharanthus roseus |
| STS | - |
Catharanthus roseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
tryptamine | wild-type strictosidine synthase expressed in yeast | Catharanthus roseus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Catharanthus roseus |
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Release 2023.1 (January 2023)
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