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Literature summary for 4.3.3.2 extracted from
- Redesign of a central enzyme in alkaloid biosynthesis (2006), Chem. Biol., 13, 1137-1141.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | redesigning the substrate specificity of strictosidine synthase is an important step toward re-engineering the TIA pathway to produce products with novel or improved biological properties | Catharanthus roseus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| for expression in Escherichia coli BL21DE3 cells | Catharanthus roseus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D177A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| E277G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| F232T | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| F314A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| F330L | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| H283A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| H313N | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| L329G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| Q282A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| S275G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| V182G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| W153F | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| Y109H | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
| Y155A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-alpha(S)-strictosidine + H2O | Catharanthus roseus | - |
tryptamine + secologanin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by one-step affinity purification using a amylose column | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-alpha(S)-strictosidine + H2O | - |
Catharanthus roseus | tryptamine + secologanin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| str | - |
Catharanthus roseus |
| strictosidine synthase | - |
Catharanthus roseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
HPLC activity assay | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
HPLC activity assay | Catharanthus roseus |
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Release 2023.1 (January 2023)
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