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Literature summary for 4.3.2.3 extracted from
- Urea is a product of ureidoglycolate degradation in chickpea. Purification and characterization of the ureidoglycolate urea-lyase (2001), Plant Physiol., 125, 828-834.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition, 50% of activity is restored after removal of EDTA, addition of Mn2+, Zn2+ or Mg2+ recover the activity to the initial levels | Cicer arietinum |  |
| Fe2+ | strong inactivation | Cicer arietinum | |
| Ni2+ | strong inactivation | Cicer arietinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
(-)-ureidoglycolate | 30°C, pH 7.8, in the presence of 0.5 mM Mn2+ | Cicer arietinum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Cicer arietinum | |
| Mn2+ | 2fold increase in activity | Cicer arietinum | |
| Zn2+ | activation | Cicer arietinum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
6 * 31000, SDS-PAGE | Cicer arietinum |
| 180000 | - |
gel filtration | Cicer arietinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-ureidoglycolate | Cicer arietinum | - |
glyoxylate + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
chickpea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, gel filtration, heat treatment, ion exchange, electrophoresis | Cicer arietinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pod | developing pods | Cicer arietinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Cicer arietinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-ureidoglycolate | - |
Cicer arietinum | glyoxylate + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 31000, SDS-PAGE | Cicer arietinum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (-)-ureidoglycolate urea-lyase | - |
Cicer arietinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
and above, chemical instability of ureidoglycolate at higher temperatures | Cicer arietinum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | approx. 30% of maximal activity at 40°C | Cicer arietinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
no loss of activity after 60 min in the presence of Mn2+ | Cicer arietinum |
| 90 | - |
complete loss of activity after 40 min in the presence of Mn2+ | Cicer arietinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Cicer arietinum |
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Release 2023.1 (January 2023)
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