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Literature summary for 4.3.2.3 extracted from

  • McIninch, J.K.; McIninch, J.D.; May, S.W.
    Catalysis, stereochemistry, and inhibition of ureidoglycolate lyase (2003), J. Biol. Chem., 278, 50091-50100.
    View publication on PubMed
Search for more literature for 4.3.2.3

Inhibitors

Inhibitors Comment Organism Structure
Acetopyruvate
-
 Burkholderia cepacia
benzoylpyruvate
-
 Burkholderia cepacia
maleamic acid
-
 Burkholderia cepacia
N-acetylphenylpyruvate
-
 Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 phenylacetyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.025
-
 cinnamoyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.028
-
 4-methoxyphenylacetyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.03
-
 1-naphtylacetyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.04
-
 3-phenylpropionyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.09
-
 benzoyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.09
-
 4'-nitrophenylacetyl-alpha-hydroxyglycine 30°C, pH 7.0 Burkholderia cepacia
0.5
-
 (S)-ureidoglycolate 30°C, pH 7.0 Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-ureidoglycolate Burkholderia cepacia
-
 glyoxylate + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel, oxamate affinity, Superose Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.92
-
-
 Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-benzoyl-alpha-hydroxyglycine 2% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + benzamide
-
 ?
(S)-cinnamoyl-alpha-hydroxyglycine 5.5% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + cinnamamide
-
 ?
(S)-ureidoglycolate
-
 Burkholderia cepacia glyoxylate + urea
-
 ?
1-naphtylacetyl-alpha-hydroxyglycine 2.3% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + 1-naphtaleneacetamide
-
 ?
3-phenylpropionyl-alpha-hydroxyglycine 1.0% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + 3-phenylpropylacetamide
-
 ?
4'-methoxyphenylacetyl-alpha-hydroxyglycine 1.3% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + 4'-methoxyphenylacetamide
-
 ?
4'-nitrophenylacetyl-alpha-hydroxyglycine 0.5% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + 4'-nitrophenylacetamide
-
 ?
benzoyl-alpha-hydroxyglycine
-
 Burkholderia cepacia glyoxylate + benzamide
-
 ?
cinnamoyl-alpha-hydroxyglycine
-
 Burkholderia cepacia glyoxylate + cinnamamide
-
 ?
phenylacetyl-alpha-hydroxyglycine 0.8% of activity with (S)-ureidoglycolate Burkholderia cepacia glyoxylate + phenylacetamide
-
 ?

Synonyms

Synonyms Comment Organism
UGL
-
 Burkholderia cepacia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000022
-
 benzoylpyruvate 30°C, pH 7.0 Burkholderia cepacia
0.00056
-
 Acetopyruvate 30°C, pH 7.0 Burkholderia cepacia
0.0012
-
 N-acetylphenylpyruvate 30°C, pH 7.0 Burkholderia cepacia
11.6
-
 maleamic acid 30°C, pH 7.0 Burkholderia cepacia