Application | Comment | Organism |
---|---|---|
medicine | generation of in vitro hybrids that mimic compound heterozygote ASL for studies on disease-associated mutant enzymes. His-tagged wild-type/non-His-tagged wild-type, His-tagged wild-type/non-His-tagged R396C, His-tagged wild-type/non-His-tagged R396H, His-tagged R194C/non-His-tagged R396C, and His-tagged L311V/non-His-tagged R396H enzyme pairs are used in various hybrids generated by denaturing pairs of enzymes in 1 M guanidinium chloride and renaturing them by removing the denaturant. The hybrids have predominant amounts of heterotetramers. Analysis of the Vmax values of the hybrids indicates that most of the subunits behave independently. However, the hybrid tetramers retain weak positive cooperativity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L311V | about 90% of wild-type actitivy | Homo sapiens |
R194C | about 100% of wild-type actitivy | Homo sapiens |
R396C | about 20% of wild-type actitivy | Homo sapiens |
R396H | about 10% of wild-type actitivy | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
4 * 57000, calculated, His-tagged protein | Homo sapiens |
228000 | - |
gel filtration, His-tagged protein | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
mutant R396H, pH 7.4, 25°C | Homo sapiens |
1.1 | - |
mutant R396H, after treatment with guanidiniumHCl, pH 7.4, 25°C | Homo sapiens |
1.6 | - |
mutant R396C, after treatment with guanidiniumHCl, pH 7.4, 25°C | Homo sapiens |
1.8 | - |
mutant R396C, pH 7.4, 25°C | Homo sapiens |
7.5 | - |
His-tagged mutant L311V, after treatment with guanidiniumHCl, pH 7.4, 25°C | Homo sapiens |
7.8 | - |
His-tagged mutant L311V, pH 7.4, 25°C | Homo sapiens |
8.3 | - |
His-tagged wild-type, after treatment with guanidiniumHCl, pH 7.4, 25°C | Homo sapiens |
8.4 | - |
wild-type, after treatment with guanidiniumHCl, pH 7.4, 25°C | Homo sapiens |
8.5 | - |
His-tagged wild-type, pH 7.4, 25°C | Homo sapiens |
8.6 | - |
wild-type, pH 7.4, 25°C | Homo sapiens |
8.7 | - |
His-tagged mutant R194C, pH 7.4, 25°C | Homo sapiens |
8.9 | - |
His-tagged mutant R194C, after treatment with guanidiniumHCl, pH 7.4, 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Nomega-L-arginino)succinate | - |
Homo sapiens | fumarate + L-arginine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57000, calculated, His-tagged protein | Homo sapiens |