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Literature summary for 4.3.2.2 extracted from

  • Ariyananda, L.d.e..Z.; Lee, P.; Antonopoulos, C.; Colman, R.F.
    Biochemical and biophysical analysis of five disease-associated human adenylosuccinate lyase mutants (2009), Biochemistry, 48, 5291-5302.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K246E the mutation is associated with adenylosuccinate lyase deficiency, the Vmax for the K246E mutant enzyme is about 2% that of the wild type enzyme, K246E exists mainly as dimer or monomer Homo sapiens
L311V the mutation is associated with adenylosuccinate lyase deficiency, the Vmax for the L311V mutant enzyme is 72% about 2% that of the wild type enzyme Homo sapiens
R194C the mutation is associated with adenylosuccinate lyase deficiency,Vmax (at 25°C) for R194C is comparable to that of wild type, R194C is rapidly inactivated at 60°C Homo sapiens
R396C the mutation is associated with adenylosuccinate lyase deficiency the Vmax for the R396C mutant enzyme is about 16% that of the wild type enzyme Homo sapiens
R396H the mutation is associated with adenylosuccinate lyase deficiency, Vmax (at 25°C) for R194C is considerably reduced and affinity for adenylosuccinate is retained Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, analytical ultracentrifugation Homo sapiens
57000
-
 4 * 57000, SDS-PAGE Homo sapiens
223000
-
 gel filtration, at 22°C Homo sapiens
227000
-
 analytical ultracentrifugation, at 37°C Homo sapiens
230000
-
 analytical ultracentrifugation, at 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Homo sapiens
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
succinyladenosine monophosphate Homo sapiens
-
 AMP + fumarate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P30566
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
succinyladenosine monophosphate
-
 Homo sapiens AMP + fumarate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 57000, SDS-PAGE Homo sapiens
homotetramer 4 * 56000, analytical ultracentrifugation Homo sapiens

Synonyms

Synonyms Comment Organism
ASL
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 wild type and mutant enzymes R396H and R396C are relatively stable at 60°C for about 4 h, the R194C mutant enzyme loses most of its activity within about 1 h of incubation at 60 °C Homo sapiens