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Literature summary for 4.3.2.2 extracted from
- Important roles of hydroxylic amino acid residues in the function of Bacillus subtilis adenylosuccinate lyase (2007), Protein Sci., 16, 441-448.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| for expression in Escherichia coli strain BL21DE3 | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S306A | conserved amino acid in all adenylosuccinate lyases, mutant for testing the involvement of the residue in the enzyme function | Bacillus subtilis |
| S94A | conserved amino acid in all adenylosuccinate lyases, mutant for testing the involvement of the residue in the enzyme function | Bacillus subtilis |
| T140A | conserved amino acid in all adenylosuccinate lyases, mutant for testing the involvement of the residue in the enzyme function | Bacillus subtilis |
| T93A | conserved amino acid in all adenylosuccinate lyases, mutant for testing the involvement of the residue in the enzyme function | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00177 | - |
adenylosuccinate | wild-type enzyme | Bacillus subtilis |  |
| 0.00224 | - |
adenylosuccinate | mutant S94A | Bacillus subtilis | |
| 0.0128 | - |
adenylosuccinate | mutant S306A | Bacillus subtilis | |
| 0.0177 | - |
adenylosuccinate | mutant T93A | Bacillus subtilis | |
| 0.0192 | - |
adenylosuccinate | mutant T140A | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 193000 | - |
determined by native polyacrylamide gel electrophoresis, mutant S93A | Bacillus subtilis |
| 194000 | - |
determined by native polyacrylamide gel electrophoresis, mutant T93A | Bacillus subtilis |
| 194800 | - |
determined by analytical ultracentrifugation, mutant T140A | Bacillus subtilis |
| 196000 | - |
determined by native polyacrylamide gel electrophoresis, wild-type enzyme | Bacillus subtilis |
| 199700 | - |
determined by analytical ultracentrifugation, wild-type enzyme | Bacillus subtilis |
| 216400 | - |
determined by analytical ultracentrifugation, mutant S306A | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | Bacillus subtilis | - |
fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
| N6-(1,2-dicarboxyethyl)AMP | Bacillus subtilis | - |
fumarate + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using nickel affinity chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
complementation of inactive mutant ASLs, maximum specific activity, T140A + H141Q, T93A + H68Q, S306A + H68Q 0% wild-type activity | Bacillus subtilis |
| 0.068 | - |
complementation of inactive mutant ASLs, maximum specific activity, T93A + H141Q, 3.0% wild-type activity | Bacillus subtilis |
| 0.096 | - |
complementation of inactive mutant ASLs, maximum specific activity, S306A + H141Q, 4.6% wild-type activity | Bacillus subtilis |
| 0.21 | - |
complementation of inactive mutant ASLs, maximum specific activity, T93A + K268Q, 10.1% wild-type activity | Bacillus subtilis |
| 0.24 | - |
complementation of inactive mutant ASLs, maximum specific activity, T140A + H68Q, 11.5% wild-type activity | Bacillus subtilis |
| 0.32 | - |
complementation of inactive mutant ASLs, maximum specific activity, S306A + K268Q, 15.4% wild-type activity | Bacillus subtilis |
| 0.32 | - |
complementation of inactive mutant ASLs, maximum specific activity, T140A + K268Q, 15.4% wild-type activity | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
Bacillus subtilis | fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
| 5-aminoimidazole-(N-succinylocarboxamide) ribotide | - |
Bacillus subtilis | 5-aminoimidazole-4-carboxamide ribotide + fumarate | - |
? | |
| adenylosuccinate | - |
Bacillus subtilis | AMP + fumarate | - |
? | |
| N6-(1,2-dicarboxyethyl)AMP | - |
Bacillus subtilis | fumarate + AMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenylosuccinate lyase | - |
Bacillus subtilis |
| ASL | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00048 | - |
adenylosuccinate | mutant T140A | Bacillus subtilis | |
| 0.00689 | - |
adenylosuccinate | mutant S306A | Bacillus subtilis | |
| 0.01 | - |
adenylosuccinate | mutant T93A | Bacillus subtilis | |
| 1.14 | - |
adenylosuccinate | mutant S94A | Bacillus subtilis | |
| 1.74 | - |
adenylosuccinate | wild-type enzyme | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Bacillus subtilis |
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