Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.2.1 extracted from

  • Premkumar, M.H.; Sule, G.; Nagamani, S.C.; Chakkalakal, S.; Nordin, A.; Jain, M.; Ruan, M.Z.; Bertin, T.; Dawson, B.; Zhang, J.; Schady, D.; Bryan, N.S.; Campeau, P.M.; Erez, A.; Lee, B.
    Argininosuccinate lyase in enterocytes protects from development of necrotizing enterocolitis (2014), Am. J. Physiol. Gastrointest. Liver Physiol., 307, G347-G354.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine manipulating the enzyme expression or activity might be of potential benefit for treatment of necrotizing enterocolitis Mus musculus
medicine manipulating the enzyme expression or activity might be of potential benefit for treatment of necrotizing enterocolitis Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information generation of enterocyte-specific knockout mice of the enzyme Asl by intercrossing Aslflox/flox mice with transgenic mice expressing Cre recombinase under the control of the villin promoter. Emgineered mice are grossly indistinguishable from their littermate Aslflox/flox controls and exhibit similar growth curves and life span. Enterocytes isolated from mutant mice demonstrate an 80% reduction in mRNA expression of the enzyme Mus musculus
additional information knock-down of enzyme Asl in IEC-6 cells using lentivirus encoding shRNA targeting the enzyme. Loss of the enzyme impairs the migration of IEC-6 cells during conditions of stress Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
enterocyte
-
Mus musculus
-
enterocyte
-
Rattus norvegicus
-
IEC-6 cell
-
Rattus norvegicus
-
ileum
-
Mus musculus
-
ileum
-
Rattus norvegicus
-
intestine intestinal segments from neonatal pups Mus musculus
-
intestine intestinal segments from neonatal pups Rattus norvegicus
-

General Information

General Information Comment Organism
malfunction loss of enterocyte-derived enzyme results in increased incidence of necrotizing enterocolitis, phenotype, overview Mus musculus
malfunction loss of enterocyte-derived enzyme results in increased incidence of necrotizing enterocolitis, phenotype, overview Rattus norvegicus
physiological function argininosuccinate lyase in enterocytes protects from development of necrotizing enterocolitis, cell-autonomous role of the enzyme. The enzyme is required to maintain a NO synthesis complex containing NO synthase, argininosuccinate synthase, HSP90, and the arginine transporter SLC7A1 Mus musculus
physiological function argininosuccinate lyase in enterocytes protects from development of necrotizing enterocolitis, cell-autonomous role of the enzyme. The enzyme is required to maintain a NO synthesis complex containing NO synthase, argininosuccinate synthase, HSP90, and the arginine transporter SLC7A1 Rattus norvegicus