Literature summary for 4.3.2.1 extracted from

Sampaleanu, L.M.; Vallee, F.; Thompson, G.D.; Howell, P.L.
Three-dimensional structure of the argininosuccinate lyase frequently complementing allele Q286R (2001), Biochemistry, 40, 15570-15580.

Search for more literature for 4.3.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion, crystal structure of Q286R mutant at 2.65 A	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Q286R	frequently complementing allele, 2% of wild-type kcat	Homo sapiens
R12Q	6% of wild-type kcat	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	N,N'-bis(tert-butoxycarbonyl)-5-[(3-aminopropyl)amino]pentan-1-ol	pH 7.6	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(Nomega-L-arginino)succinate	Homo sapiens	-	fumarate + L-arginine	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	mutations in ASL result in the clinical condition argininosuccinic aciduria, an autosomal recessive disorder	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant ASL	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(Nomega-L-arginino)succinate	-	Homo sapiens	fumarate + L-arginine	-	?
(Nomega-L-arginino)succinate	-	Homo sapiens	fumarate + L-arginine	-	r
N,N'-bis(tert-butoxycarbonyl)-5-[(3-aminopropyl)amino]pentan-1-ol	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
ASL	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
34.5	-	N,N'-bis(tert-butoxycarbonyl)-5-[(3-aminopropyl)amino]pentan-1-ol	pH 7.6	Homo sapiens

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Release 2023.1 (January 2023)