Cloned (Comment) | Organism |
---|---|
Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-1-amino-2-propanol | inactive substrate analogue | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | a dissociation constant for coenzyme B12 binding to EAL of 0.0005 mM is estimated from the Michaelis constant, which indicates that the free cofactor represents 4% of the total cofactor in the sample | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | substrate binding is required for cobalt-carbon bond cleavage in the native system. | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of the purified enzyme with aminoethanol as the substrate is 35-45 micromol/min/mg, determined by using the coupled assay with alcohol dehydrogenase/NADH | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-amino-2-propanol | inactive substrate analogue, which binds to the substrate binding site in EAL but does not form the cob(II)alamin-substrate radical pair state | Salmonella enterica subsp. enterica serovar Typhimurium | propanal + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ethanolamine ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | adenosylcobalamin | Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
(S)-1-amino-2-propanol | dissociation constant for interaction with holo-EAL | Salmonella enterica subsp. enterica serovar Typhimurium |