General Stability | Organism |
---|---|
rapid decrease of activity in presence of mercaptoethanol or cysteine, 56% of initial activity after 1 d, 20% of initial activity after 2 d. In presence of DTT, the enzyme remains stable for several days | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co3+ | 0.1 mM, 95% inhibition | Chlamydomonas reinhardtii | |
Cu2+ | 0.1 mM, 70% inhibition | Chlamydomonas reinhardtii | |
cyanide | 50 mM histidinol phosphate, 5 mM L-histidine and 5 mM Mn2+ protects | Chlamydomonas reinhardtii | |
Fe2+ | 0.1 mM, 60% inhibition | Chlamydomonas reinhardtii | |
Fe3+ | 0.1 mM, 60% inhibition | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme does not exhibit classical Michaelis-Menten kinetics | Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.1 mM, activation to 124.5% of control | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38100 | - |
4 * 38100, SDS-PAGE | Chlamydomonas reinhardtii |
152400 | - |
gel filtration | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
CCAP 11/32A | - |
Chlamydomonas reinhardtii CCAP 11/32A | - |
CCAP 11/32A | - |
Purification (Comment) | Organism |
---|---|
- |
Chlamydomonas reinhardtii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | grown axenically at 18°C under continous illumination. Cells grown on ammonium as the sole nitrogen source contain no histidase activity, cells grown on histidine, on histidine and glycine, or on histidine and aspartate contain histidase and exhibited similar histidase activities. When the cells are grown on histidine and ammonium, or on histidine and succinate, or on histidine and glutamate, the production of histidase is slightly delayed and reaches a lower level than when cells were grown on histidine as the sole source of nitrogen. No histidase activity was detected when C. reinhardtii was grown in the presence of acetate | Chlamydomonas reinhardtii | - |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38100, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.2 | - |
Chlamydomonas reinhardtii |